{"id":"05504d0c-2dba-59fc-8a13-3aac7e92c3da","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-ado-calcium-signals","predicate":"modulates","statement":"Manipulating ADO altered G-protein-coupled calcium signals and MAP-kinase activity in the studied human cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a588f559-10c7-59d5-9ce7-e48110a3d100","mechanism_event_label":"Protein turnover connected an oxygen-sensing step to signaling outputs.","subject":{"id":"fdeeed42-76ed-5016-b0bc-c1bf9120af3d","slug":"ado","display_name":"Human 2-aminoethanethiol dioxygenase / ADO","entity_type_key":"protein"},"object":{"id":"b90a53f5-612a-5ef2-b240-970f030b9768","slug":"human-ado-gpcr-calcium-mapk","display_name":"G-protein-coupled calcium and MAPK signals in human ADO experiments","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a588f559-10c7-59d5-9ce7-e48110a3d100","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-ado-calcium-signals-event","event_type":"observed_relationship","label":"Protein turnover connected an oxygen-sensing step to signaling outputs.","description":"Manipulating ADO altered G-protein-coupled calcium signals and MAP-kinase activity in the studied human cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fdeeed42-76ed-5016-b0bc-c1bf9120af3d","slug":"ado","display_name":"Human 2-aminoethanethiol dioxygenase / ADO","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b90a53f5-612a-5ef2-b240-970f030b9768","slug":"human-ado-gpcr-calcium-mapk","display_name":"G-protein-coupled calcium and MAPK signals in human ADO experiments","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f52e36ad-972a-52c2-b8e9-1cbacffeedbc","slug":"protein-n-terminal-cysteine","display_name":"Protein-incorporated N-terminal cysteine residue","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human-cell functional signaling assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence of dietary calcium or cysteine requirements for this response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Cysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Protein turnover connected an oxygen-sensing step to signaling outputs.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Conserved N-terminal cysteine dioxygenases transduce responses to hypoxia in animals and plants. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31273118/ · DOI 10.1126/science.aaw0112","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"37326d7c-1b2d-596e-8a02-31fd10ac7b0a","evidence_kind":"source_excerpt","locator":"Lines 436-442","start_line":436,"end_line":442,"excerpt":"## l-cysteine-ado-calcium-signals\nProtein turnover connected an oxygen-sensing step to signaling outputs.\nManipulating ADO altered G-protein-coupled calcium signals and MAP-kinase activity in the studied human cells.\nModel: Human-cell functional signaling assays.\nLimitations: Not evidence of dietary calcium or cysteine requirements for this response.\nEvidence access: Primary full text\nConserved N-terminal cysteine dioxygenases transduce responses to hypoxia in animals and plants. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31273118/ · DOI 10.1126/science.aaw0112","model_system":"Human-cell functional signaling assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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