{"id":"0535d0e1-f903-5469-8520-e1b9c4594d09","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-calcium-sensitivity","predicate":"increases","statement":"Adding 8 or 16 mM carnosine increased contractile-apparatus calcium sensitivity in mechanically skinned human type I and II muscle fibers.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"128a5827-dbc0-564e-8a17-8c341217da37","mechanism_event_label":"Muscle fibers responded more strongly to the same calcium signal.","subject":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"object":{"id":"e12a13e8-c381-5723-ad4b-a6d3b994b776","slug":"human-muscle-calcium-sensitivity","display_name":"Human muscle-fiber contractile calcium sensitivity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"128a5827-dbc0-564e-8a17-8c341217da37","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-calcium-sensitivity-event","event_type":"observed_relationship","label":"Muscle fibers responded more strongly to the same calcium signal.","description":"Adding 8 or 16 mM carnosine increased contractile-apparatus calcium sensitivity in mechanically skinned human type I and II muscle fibers.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e12a13e8-c381-5723-ad4b-a6d3b994b776","slug":"human-muscle-calcium-sensitivity","display_name":"Human muscle-fiber contractile calcium sensitivity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human vastus lateralis biopsy fibers ex vivo.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Bath exposure does not establish an oral supplementation effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Carnosine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Muscle fibers responded more strongly to the same calcium signal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Effects of carnosine on contractile apparatus Ca²⁺ sensitivity and sarcoplasmic reticulum Ca²⁺ release in human skeletal muscle fibers. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22174397/ · DOI 10.1152/japplphysiol.01331.2011","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cd6c33cb-6461-5079-b8aa-0ff81191497b","evidence_kind":"source_excerpt","locator":"Lines 188-194","start_line":188,"end_line":194,"excerpt":"## carnosine-calcium-sensitivity\nMuscle fibers responded more strongly to the same calcium signal.\nAdding 8 or 16 mM carnosine increased contractile-apparatus calcium sensitivity in mechanically skinned human type I and II muscle fibers.\nModel: Human vastus lateralis biopsy fibers ex vivo.\nLimitations: Bath exposure does not establish an oral supplementation effect.\nEvidence access: Primary abstract\nEffects of carnosine on contractile apparatus Ca²⁺ sensitivity and sarcoplasmic reticulum Ca²⁺ release in human skeletal muscle fibers. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22174397/ · DOI 10.1152/japplphysiol.01331.2011","model_system":"Human vastus lateralis biopsy fibers ex vivo.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"87dc5af2-4eb6-5980-b7e0-bf3697b1156e","stable_key":"import-ae1ed35d-8ce7-5936-9067-a641374bbf79","title":"Carnosine: synthesis, transport, carbonyl chemistry and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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