{"id":"051a6cc4-bd64-5fa0-994e-15a7e9164644","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-chdh-motility","predicate":"reduces","statement":"Chdh-null males produced comparable amounts of sperm but had diminished sperm motility.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e2feffcf-cd03-53f4-999d-8c021df0fd49","mechanism_event_label":"Sperm number and sperm movement were different outcomes.","subject":{"id":"78c8e1df-b5f8-5bd0-8d35-503d3e89a175","slug":"mouse-chdh-null","display_name":"Experimental mouse Chdh-null genotype","entity_type_key":"protein_state"},"object":{"id":"da652899-3d54-52c0-9802-a7e3e84efb97","slug":"mouse-sperm-motility","display_name":"Mouse sperm motility","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e2feffcf-cd03-53f4-999d-8c021df0fd49","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-chdh-motility-event","event_type":"biochemical_relationship","label":"Sperm number and sperm movement were different outcomes.","description":"Chdh-null males produced comparable amounts of sperm but had diminished sperm motility.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bb913152-b288-5398-9c04-2345f3a4202b","slug":"mouse-chdh","display_name":"Mouse choline dehydrogenase / Chdh","entity_type_key":"protein"},"role":"missing_enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"78c8e1df-b5f8-5bd0-8d35-503d3e89a175","slug":"mouse-chdh-null","display_name":"Experimental mouse Chdh-null genotype","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"da652899-3d54-52c0-9802-a7e3e84efb97","slug":"mouse-sperm-motility","display_name":"Mouse sperm motility","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/20371614.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"dce0c14d2eb09194040087e691053960d9e4f477a49099cdd9482ad9ab38dc48\", \"start_char\": 0, \"end_char\": 1334, \"text_sha256\": \"dce0c14d2eb09194040087e691053960d9e4f477a49099cdd9482ad9ab38dc48\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Chdh-null mouse genetics and sperm biochemical assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Chdh deletion versus wild type","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Loss of an enzyme is not the same exposure as low dietary choline; mouse fertility results are not a human diagnostic rule. The 2018 correction replaces the original knockout exon diagram: exons 1–6 and much of exon 7 were deleted, not only exons 1–3 (PMID 29698116; doi:10.1096/fj.09-153718ERR).","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Choline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Sperm number and sperm movement were different outcomes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[choline-p20371614] Deletion of murine choline dehydrogenase results in diminished sperm motility. (2010). https://pubmed.ncbi.nlm.nih.gov/20371614/ DOI: 10.1096/fj.09-153718","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Testis and sperm mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"d2fff083-f3bb-5579-a147-55ec985501dd","evidence_kind":"source_excerpt","locator":"Lines 594-605","start_line":594,"end_line":605,"excerpt":"### choline-chdh-motility\nChdh-null males produced comparable amounts of sperm but had diminished sperm motility.\nCondition category: machinery_impairment\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Sperm number and sperm movement were different outcomes.\norganism: Mouse\ntissue_or_cell_type: Testis and sperm mitochondria\nexperimental_model: Chdh-null mouse genetics and sperm biochemical assays\nlimitations: Loss of an enzyme is not the same exposure as low dietary choline; mouse fertility results are not a human diagnostic rule. The 2018 correction replaces the original knockout exon diagram: exons 1–6 and much of exon 7 were deleted, not only exons 1–3 (PMID 29698116; doi:10.1096/fj.09-153718ERR).\nexposure: Chdh deletion versus wild type\nevidence_span: {\"source_cache\": \"artifacts/choline-research/20371614.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"dce0c14d2eb09194040087e691053960d9e4f477a49099cdd9482ad9ab38dc48\", \"start_char\": 0, \"end_char\": 1334, \"text_sha256\": \"dce0c14d2eb09194040087e691053960d9e4f477a49099cdd9482ad9ab38dc48\"}\n[choline-p20371614] Deletion of murine choline dehydrogenase results in diminished sperm motility. 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