{"id":"050c83a7-8196-5bf6-a741-3b17c7b242e5","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-nuclear-propionyl","predicate":"supplies","statement":"Isotope tracing and controlled subcellular acyl-CoA measurement identified isoleucine as a major source of nuclear propionyl-CoA and histone propionyl groups in the tested cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7e495ae9-6da9-506c-ab54-117121e620fb","mechanism_event_label":"An amino-acid carbon skeleton can reach a chromatin modification.","subject":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"object":{"id":"af2bb5c5-08c3-57cf-a43e-b393bb55f009","slug":"cancer-cell-nuclear-propionyl-coa","display_name":"Nuclear propionyl-CoA in studied cancer cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7e495ae9-6da9-506c-ab54-117121e620fb","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-nuclear-propionyl-event","event_type":"observed_relationship","label":"An amino-acid carbon skeleton can reach a chromatin modification.","description":"Isotope tracing and controlled subcellular acyl-CoA measurement identified isoleucine as a major source of nuclear propionyl-CoA and histone propionyl groups in the tested cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"af2bb5c5-08c3-57cf-a43e-b393bb55f009","slug":"cancer-cell-nuclear-propionyl-coa","display_name":"Nuclear propionyl-CoA in studied cancer cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"69ac0570-94d8-59d8-85ef-7f67cf302d16","slug":"propionyl-coa","display_name":"Propionyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"SILEC-SF fractionation/tracing; 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specific cell lines and exposure not resolved in accessed abstract.\nLimitations: Not a claim that all tissues use the same carbon source or that oral isoleucine controls human gene expression.\nEvidence access: Primary abstract\nQuantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34856123/ · DOI 10.1016/j.molcel.2021.11.006","model_system":"SILEC-SF fractionation/tracing; specific cell lines and exposure not resolved in accessed abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4b00f118-00fd-5cef-be97-13125eb958e9","stable_key":"import-09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2","title":"L-Isoleucine: transport, translation, catabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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