{"id":"04f54d9c-a0dd-575b-831e-508bd68cdf6d","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:a-vision-rpe65-isomerization","predicate":"produces","statement":"Recombinant RPE65 catalyzed conversion of all-trans-retinyl esters to 11-cis-retinol in visual-cycle reconstitution.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"3e15ecf2-689e-585e-8aa5-f6263af39923","mechanism_event_label":"RPE65 converts the ester pool into the cis alcohol needed for chromophore renewal.","subject":{"id":"4b5339c8-6f83-5d4c-a3b4-79c3c440c23d","slug":"rpe65","display_name":"RPE65 retinoid isomerohydrolase","entity_type_key":"protein"},"object":{"id":"4a978267-139b-518d-bdc6-e16cda6f5225","slug":"11-cis-retinol","display_name":"11-cis-retinol","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"3e15ecf2-689e-585e-8aa5-f6263af39923","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:a-vision-rpe65-isomerization-event","event_type":"biochemical_relationship","label":"RPE65 converts the ester pool into the cis alcohol needed for chromophore renewal.","description":"Recombinant RPE65 catalyzed conversion of all-trans-retinyl esters to 11-cis-retinol in visual-cycle reconstitution.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dd021e86-1cb4-5448-8cb4-879b7a7f1052","slug":"all-trans-retinyl-esters","display_name":"All-trans-retinyl esters","entity_type_key":"chemical_species"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f7ce5851-df6d-54ed-b467-482480d26ff9","slug":"lrat","display_name":"Lecithin:retinol acyltransferase / LRAT","entity_type_key":"protein"},"role":"ester supply enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4b5339c8-6f83-5d4c-a3b4-79c3c440c23d","slug":"rpe65","display_name":"RPE65 retinoid isomerohydrolase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4a978267-139b-518d-bdc6-e16cda6f5225","slug":"11-cis-retinol","display_name":"11-cis-retinol","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Recombinant enzyme reconstitution","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Production of the aldehyde requires a subsequent oxidation step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"QBI-293A human cells and COS-1 monkey cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"RPE65 converts the ester pool into the cis alcohol needed for chromophore renewal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[moiseyev-2005] RPE65 is the isomerohydrolase in the retinoid visual cycle (2005). https://pubmed.ncbi.nlm.nih.gov/16116091/ DOI: 10.1073/pnas.0503460102","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"RPE visual-cycle biochemical model","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d6ce5916-236f-5ee1-b040-40233df19fe8","evidence_kind":"source_excerpt","locator":"Lines 666-675","start_line":666,"end_line":675,"excerpt":"### a-vision-rpe65-isomerization\nRecombinant RPE65 catalyzed conversion of all-trans-retinyl esters to 11-cis-retinol in visual-cycle reconstitution.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: RPE65 converts the ester pool into the cis alcohol needed for chromophore renewal.\norganism: QBI-293A human cells and COS-1 monkey cells\ntissue_or_cell_type: RPE visual-cycle biochemical model\nexperimental_model: Recombinant enzyme reconstitution\nlimitations: Production of the aldehyde requires a subsequent oxidation step.\n[moiseyev-2005] RPE65 is the isomerohydrolase in the retinoid visual cycle (2005). https://pubmed.ncbi.nlm.nih.gov/16116091/ DOI: 10.1073/pnas.0503460102","model_system":"Recombinant enzyme reconstitution","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [moiseyev-2005] RPE65 is the isomerohydrolase in the retinoid visual cycle (2005). https://pubmed.ncbi.nlm.nih.gov/16116091/ DOI: 10.1073/pnas.0503460102","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0c5474e2-be48-547b-b287-9c07a59c9ff1","stable_key":"import-dff9f743-3766-5f20-a71e-d6d2bd5bfb6f","title":"Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b217224b262abbd77d6a0c8c9beefff9c022fae4d31e2a5273915d769b9a6546","revision_id":"61008909-871f-575c-b5e2-21542689ea3c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}