{"id":"04f30f6a-cf7b-55d0-8f76-56f146bac728","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-fasting-human","predicate":"associated_with_increase","statement":"PBMC hypusinated eIF5A increased during the studied multiday fasting regimen and remained elevated after food reintroduction.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7fe7ce80-cb00-5774-932d-a47089a66224","mechanism_event_label":"Human blood cells showed a pathway response.","subject":{"id":"f7005c47-f651-5be7-974f-17f7956941d9","slug":"human-prolonged-fasting-exposure","display_name":"Human prolonged-fasting study exposure","entity_type_key":"cellular_process"},"object":{"id":"0a81f1c8-29ba-504f-97f2-ac8557b65396","slug":"human-pbmc-eif5a-hypusination","display_name":"Human PBMC eIF5A hypusination","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7fe7ce80-cb00-5774-932d-a47089a66224","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-fasting-human-event","event_type":"observed_relationship","label":"Human blood cells showed a pathway response.","description":"PBMC hypusinated eIF5A increased during the studied multiday fasting regimen and remained elevated after food reintroduction.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f7005c47-f651-5be7-974f-17f7956941d9","slug":"human-prolonged-fasting-exposure","display_name":"Human prolonged-fasting study exposure","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0a81f1c8-29ba-504f-97f2-ac8557b65396","slug":"human-pbmc-eif5a-hypusination","display_name":"Human PBMC eIF5A hypusination","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Healthy human volunteers sampled during fasting.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a causal test of human longevity or a recommendation for prolonged fasting.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Human blood cells showed a pathway response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Spermidine is essential for fasting-mediated autophagy and longevity. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39117797/ · DOI 10.1038/s41556-024-01468-x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9c1e72b9-2355-57d0-a0e9-3a29f4a342c7","evidence_kind":"source_excerpt","locator":"Lines 206-212","start_line":206,"end_line":212,"excerpt":"## spermidine-fasting-human\nHuman blood cells showed a pathway response.\nPBMC hypusinated eIF5A increased during the studied multiday fasting regimen and remained elevated after food reintroduction.\nModel: Healthy human volunteers sampled during fasting.\nLimitations: Not a causal test of human longevity or a recommendation for prolonged fasting.\nEvidence access: Primary full text\nSpermidine is essential for fasting-mediated autophagy and longevity. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39117797/ · DOI 10.1038/s41556-024-01468-x","model_system":"Healthy human volunteers sampled during fasting.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}