{"id":"04e00143-7e1c-5956-a4be-2398619cf546","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-pufferfish-transporter","predicate":"mediates","statement":"Pufferfish Slc4a11A expression increased boron accumulation, intracellular pH and outward currents during boric-acid exposure in oocytes, independently of extracellular sodium.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"04094803-2d8e-55db-a761-0c5a5f13a769","mechanism_event_label":"A fish version really did transport boric-acid equivalents in this experiment; that does not validate the disputed human assignment.","subject":{"id":"c59d5923-f7b0-555e-8ccb-e74e3000614b","slug":"pufferfish-slc4a11a","display_name":"Takifugu obscurus Slc4a11A","entity_type_key":"protein"},"object":{"id":"53f200cf-f2e3-506e-8c7a-c9981d3897e5","slug":"cellular-boric-acid-transport","display_name":"Cellular boric-acid-equivalent transport","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"04094803-2d8e-55db-a761-0c5a5f13a769","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-pufferfish-transporter-event","event_type":"biochemical_relationship","label":"A fish version really did transport boric-acid equivalents in this experiment; that does not validate the disputed human assignment.","description":"Pufferfish Slc4a11A expression increased boron accumulation, intracellular pH and outward currents during boric-acid exposure in oocytes, independently of extracellular sodium.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c59d5923-f7b0-555e-8ccb-e74e3000614b","slug":"pufferfish-slc4a11a","display_name":"Takifugu obscurus Slc4a11A","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"53f200cf-f2e3-506e-8c7a-c9981d3897e5","slug":"cellular-boric-acid-transport","display_name":"Cellular boric-acid-equivalent transport","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/36435196.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f33e845c95cab0ebe899c99562ebb23f43292cbab645601a5ab51a421fdc5f8c\", \"start_char\": 0, \"end_char\": 1651, \"text_sha256\": \"f33e845c95cab0ebe899c99562ebb23f43292cbab645601a5ab51a421fdc5f8c\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Freshwater/seawater acclimation and Xenopus oocyte transporter expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Freshwater versus seawater; boric-acid exposure during voltage clamp","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Fish paralog is distinct from human SLC4A11. Borate uniport, boric-acid/OH cotransport and boric-acid/H exchange were not distinguished.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Boron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Takifugu obscurus pufferfish; Xenopus expression host","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A fish version really did transport boric-acid equivalents in this experiment; that does not validate the disputed human assignment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[boron-p36435196] Seawater fish use an electrogenic boric acid transporter, Slc4a11A, for boric acid excretion by the kidney. (2023). https://pubmed.ncbi.nlm.nih.gov/36435196/ DOI: 10.1016/j.jbc.2022.102740","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Kidney tubular apical membrane and heterologous membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3aa9be10-7bf7-55c9-8aaa-9407c0cf9859","evidence_kind":"source_excerpt","locator":"Lines 248-259","start_line":248,"end_line":259,"excerpt":"### boron-pufferfish-transporter\nPufferfish Slc4a11A expression increased boron accumulation, intracellular pH and outward currents during boric-acid exposure in oocytes, independently of extracellular sodium.\nCondition category: normal\nnutrient_topic: Boron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A fish version really did transport boric-acid equivalents in this experiment; that does not validate the disputed human assignment.\norganism: Takifugu obscurus pufferfish; Xenopus expression host\ntissue_or_cell_type: Kidney tubular apical membrane and heterologous membrane\nexperimental_model: Freshwater/seawater acclimation and Xenopus oocyte transporter expression\nlimitations: Fish paralog is distinct from human SLC4A11. Borate uniport, boric-acid/OH cotransport and boric-acid/H exchange were not distinguished.\nexposure: Freshwater versus seawater; boric-acid exposure during voltage clamp\nevidence_span: {\"source_cache\": \"artifacts/boron-research/36435196.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f33e845c95cab0ebe899c99562ebb23f43292cbab645601a5ab51a421fdc5f8c\", \"start_char\": 0, \"end_char\": 1651, \"text_sha256\": \"f33e845c95cab0ebe899c99562ebb23f43292cbab645601a5ab51a421fdc5f8c\"}\n[boron-p36435196] Seawater fish use an electrogenic boric acid transporter, Slc4a11A, for boric acid excretion by the kidney. (2023). https://pubmed.ncbi.nlm.nih.gov/36435196/ DOI: 10.1016/j.jbc.2022.102740","model_system":"Freshwater/seawater acclimation and Xenopus oocyte transporter expression","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [boron-p36435196] Seawater fish use an electrogenic boric acid transporter, Slc4a11A, for boric acid excretion by the kidney. (2023). https://pubmed.ncbi.nlm.nih.gov/36435196/ DOI: 10.1016/j.jbc.2022.102740","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2d96c547-aeec-5d8b-9d31-1288da209f89","stable_key":"import-7edf94bb-95c8-5234-9161-9eb338bb9b36","title":"Boron: chemistry, nutrient interactions, low-intake studies and mechanistic uncertainties (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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