{"id":"04be5c8d-cecb-5257-9bf5-27b56d651619","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-pineal-calcium","predicate":"increases_tested","statement":"D-aspartate at 1 mM increased intracellular calcium in 11 of 13 tested rat pinealocytes; NMDA, AMPA and kainate did not evoke this response in the reported panels.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d61d436e-b6a5-5c93-9081-95246e942472","mechanism_event_label":"This pineal response differs from ordinary neuronal NMDA-receptor activation.","subject":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"object":{"id":"3078f0ee-56af-5480-b0a0-b0d6d108dd50","slug":"rat-pineal-calcium-entry","display_name":"Calcium entry in rat pinealocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d61d436e-b6a5-5c93-9081-95246e942472","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-pineal-calcium-event","event_type":"observed_relationship","label":"This pineal response differs from ordinary neuronal NMDA-receptor activation.","description":"D-aspartate at 1 mM increased intracellular calcium in 11 of 13 tested rat pinealocytes; 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many detailed blocker experiments used L-aspartate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"This pineal response differs from ordinary neuronal NMDA-receptor activation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Glutamate transporter-mediated glutamate secretion in the mammalian pineal gland. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18945893/ · DOI 10.1523/JNEUROSCI.0894-08.2008","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e177066e-5ed3-55db-ac84-2d65b8e47185","evidence_kind":"source_excerpt","locator":"Lines 160-166","start_line":160,"end_line":166,"excerpt":"## d-aspartate-pineal-calcium\nThis pineal response differs from ordinary neuronal NMDA-receptor activation.\nD-aspartate at 1 mM increased intracellular calcium in 11 of 13 tested rat pinealocytes; NMDA, AMPA and kainate did not evoke this response in the reported panels.\nModel: Rat pinealocyte Fura-2 imaging with substrate/agonist comparisons.\nLimitations: Transporter depolarization and voltage-gated calcium entry explain the model; many detailed blocker experiments used L-aspartate.\nEvidence access: Primary full text\nGlutamate transporter-mediated glutamate secretion in the mammalian pineal gland. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18945893/ · DOI 10.1523/JNEUROSCI.0894-08.2008","model_system":"Rat pinealocyte Fura-2 imaging with substrate/agonist comparisons.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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