{"id":"04ad4247-2b13-5c46-808e-ae2df8d05d16","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-pkc-block-null","predicate":"does_not_block","statement":"PKC inhibition or downregulation, alone or combined with wortmannin, failed to block vanadate/pervanadate-stimulated glucose transport.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"71574133-66b6-5ded-bf13-4b74dbbb6132","mechanism_event_label":"Blocking another signaling family did not remove the effect in these cells.","subject":{"id":"2ccb2232-a697-5152-ad30-6b002f3722c3","slug":"rat-muscle-pkc-inhibition","display_name":"PKC inhibition or downregulation in rat muscle cells","entity_type_key":"cellular_process"},"object":{"id":"6efe3403-195d-5d5c-b94e-5df545d4a772","slug":"rat-l6-vanadate-glucose-transport","display_name":"Glucose transport in vanadate-treated rat L6 myotubes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"71574133-66b6-5ded-bf13-4b74dbbb6132","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-pkc-block-null-event","event_type":"observed_relationship","label":"Blocking another signaling family did not remove the effect in these cells.","description":"PKC inhibition or downregulation, alone or combined with wortmannin, failed to block vanadate/pervanadate-stimulated glucose transport.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2ccb2232-a697-5152-ad30-6b002f3722c3","slug":"rat-muscle-pkc-inhibition","display_name":"PKC inhibition or downregulation in rat muscle cells","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6efe3403-195d-5d5c-b94e-5df545d4a772","slug":"rat-l6-vanadate-glucose-transport","display_name":"Glucose transport in vanadate-treated rat L6 myotubes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f1faad21-091c-5b03-a03c-a20011d36fef","slug":"pervanadate","display_name":"Pervanadate / peroxovanadium(V) assay species","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat L6 inhibitor and prolonged-PMA experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No conclusion about every PKC isoform in other cell types.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Blocking another signaling family did not remove the effect in these cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Tyrosine phosphatase inhibitors, vanadate and pervanadate, stimulate glucose transport and GLUT translocation in muscle cells by a mechanism independent of phosphatidylinositol 3-kinase and protein kinase C. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9792535/ · DOI 10.2337/diabetes.47.11.1676","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9406091a-5c21-52c3-8441-188e0a3c0258","evidence_kind":"source_excerpt","locator":"Lines 86-92","start_line":86,"end_line":92,"excerpt":"## vanadium-pkc-block-null\nBlocking another signaling family did not remove the effect in these cells.\nPKC inhibition or downregulation, alone or combined with wortmannin, failed to block vanadate/pervanadate-stimulated glucose transport.\nModel: Rat L6 inhibitor and prolonged-PMA experiments.\nLimitations: No conclusion about every PKC isoform in other cell types.\nEvidence access: Primary abstract\nTyrosine phosphatase inhibitors, vanadate and pervanadate, stimulate glucose transport and GLUT translocation in muscle cells by a mechanism independent of phosphatidylinositol 3-kinase and protein kinase C. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9792535/ · DOI 10.2337/diabetes.47.11.1676","model_system":"Rat L6 inhibitor and prolonged-PMA experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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