{"id":"04a52617-183a-5f2e-800d-dde3324b21bd","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-trans-slc30a10-d40a-transport-loss","predicate":"impairs","statement":"Human SLC30A10 D40A lost manganese transport function in the HEK293T assay compared with wild-type SLC30A10.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"41fe0d7a-ab03-57a8-a630-a9b74e9d895d","mechanism_event_label":"Changing aspartate 40 to alanine disabled the measured manganese transport function.","subject":{"id":"90a3daa1-c63a-5c64-b5ef-665aef13d3ab","slug":"slc30a10-d40a","display_name":"Human SLC30A10 D40A mutant","entity_type_key":"protein_state"},"object":{"id":"501b2e42-1906-57d6-9c0d-fb59de07149a","slug":"cellular-manganese-efflux","display_name":"Cellular manganese efflux","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"41fe0d7a-ab03-57a8-a630-a9b74e9d895d","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-trans-slc30a10-d40a-transport-loss-event","event_type":"biochemical_relationship","label":"Changing aspartate 40 to alanine disabled the measured manganese transport function.","description":"Human SLC30A10 D40A lost manganese transport function in the HEK293T assay compared with wild-type SLC30A10.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5ba76eba-65ec-5f9c-a42d-dad58f6633d4","slug":"slc30a10","display_name":"Human manganese exporter SLC30A10","entity_type_key":"protein"},"role":"wild_type_comparator","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"90a3daa1-c63a-5c64-b5ef-665aef13d3ab","slug":"slc30a10-d40a","display_name":"Human SLC30A10 D40A mutant","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"501b2e42-1906-57d6-9c0d-fb59de07149a","slug":"cellular-manganese-efflux","display_name":"Cellular manganese efflux","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Site-directed SLC30A10 mutagenesis in HEK293T cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"D40A versus wild-type human SLC30A10 expression in HEK293T cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The functional mutant comparison does not establish treatment efficacy or a dietary manganese deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Manganese research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"manganese","display_name":"Manganese","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Changing aspartate 40 to alanine disabled the measured manganese transport function.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mn-trans-41022720] Molecular mechanisms of SLC30A10-mediated manganese transport. (2025). https://pubmed.ncbi.nlm.nih.gov/41022720/ DOI: 10.1038/s41467-025-63616-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK293T cellular manganese handling","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"467064b8-72a2-5398-98cc-4b22fbb2f732","evidence_kind":"source_excerpt","locator":"Lines 409-420","start_line":409,"end_line":420,"excerpt":"### mn-trans-slc30a10-d40a-transport-loss\nHuman SLC30A10 D40A lost manganese transport function in the HEK293T assay compared with wild-type SLC30A10.\nCondition category: machinery_impairment\nnutrient_topic: Manganese research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Changing aspartate 40 to alanine disabled the measured manganese transport function.\norganism: Homo sapiens\ntissue_or_cell_type: HEK293T cellular manganese handling\nexperimental_model: Site-directed SLC30A10 mutagenesis in HEK293T cells\nlimitations: The functional mutant comparison does not establish treatment efficacy or a dietary manganese deficiency.\nexposure: D40A versus wild-type human SLC30A10 expression in HEK293T cells.\ncross_nutrient: false\n[mn-trans-41022720] Molecular mechanisms of SLC30A10-mediated manganese transport. (2025). https://pubmed.ncbi.nlm.nih.gov/41022720/ DOI: 10.1038/s41467-025-63616-7","model_system":"Site-directed SLC30A10 mutagenesis in HEK293T cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mn-trans-41022720] Molecular mechanisms of SLC30A10-mediated manganese transport. (2025). https://pubmed.ncbi.nlm.nih.gov/41022720/ DOI: 10.1038/s41467-025-63616-7","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"03224387-8a73-5b4f-906d-9f0be6625b7f","stable_key":"import-be889add-cec8-500b-be89-676431432a70","title":"Manganese: enzyme cofactors, glycosylation, transport and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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