{"id":"047676d5-1180-537e-91cc-80c26b835eb1","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-isyna-zinc","predicate":"inhibits","statement":"Zn2+ strongly inhibited purified human ISYNA1 in the cation comparison.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"8f37e850-0c3c-50ba-95ef-54ce84534674","mechanism_event_label":"A mineral can inhibit one enzyme even while supporting many other functions.","subject":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"object":{"id":"bab0b109-e18f-5fe1-8631-364242ec78f4","slug":"isyna1","display_name":"ISYNA1 (human inositol-3-phosphate synthase 1)","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"8f37e850-0c3c-50ba-95ef-54ce84534674","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-isyna-zinc-event","event_type":"biochemical_relationship","label":"A mineral can inhibit one enzyme even while supporting many other functions.","description":"Zn2+ strongly inhibited purified human ISYNA1 in the cation comparison.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bab0b109-e18f-5fe1-8631-364242ec78f4","slug":"isyna1","display_name":"ISYNA1 (human inositol-3-phosphate synthase 1)","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/15024000.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5ab3a8eda77989c3e4a681b4a971fb7e969c285d3e5c8e41bc31713a7f88c9ac\", \"start_char\": 0, \"end_char\": 1427, \"text_sha256\": \"5ab3a8eda77989c3e4a681b4a971fb7e969c285d3e5c8e41bc31713a7f88c9ac\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human enzyme and complementation of yeast ino1 deletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Substrate, NAD+, cation and valproate experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified-enzyme and yeast results do not establish supplement effects or a human dietary deficiency threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human protein expressed in bacteria and yeast","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A mineral can inhibit one enzyme even while supporting many other functions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p15024000] Human 1-D-myo-inositol-3-phosphate synthase is functional in yeast. (2004). https://pubmed.ncbi.nlm.nih.gov/15024000/ DOI: 10.1074/jbc.m312078200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified enzyme; yeast cultures","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"90397378-ca1d-5369-b8fb-a803ab2f00c4","evidence_kind":"source_excerpt","locator":"Lines 210-221","start_line":210,"end_line":221,"excerpt":"### ino-isyna-zinc\nZn2+ strongly inhibited purified human ISYNA1 in the cation comparison.\nCondition category: normal\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A mineral can inhibit one enzyme even while supporting many other functions.\norganism: Human protein expressed in bacteria and yeast\ntissue_or_cell_type: Purified enzyme; yeast cultures\nexperimental_model: Recombinant human enzyme and complementation of yeast ino1 deletion\nlimitations: Purified-enzyme and yeast results do not establish supplement effects or a human dietary deficiency threshold.\nexposure: Substrate, NAD+, cation and valproate experiments\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/15024000.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5ab3a8eda77989c3e4a681b4a971fb7e969c285d3e5c8e41bc31713a7f88c9ac\", \"start_char\": 0, \"end_char\": 1427, \"text_sha256\": \"5ab3a8eda77989c3e4a681b4a971fb7e969c285d3e5c8e41bc31713a7f88c9ac\"}\n[ino-p15024000] Human 1-D-myo-inositol-3-phosphate synthase is functional in yeast. (2004). https://pubmed.ncbi.nlm.nih.gov/15024000/ DOI: 10.1074/jbc.m312078200","model_system":"Recombinant human enzyme and complementation of yeast ino1 deletion","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p15024000] Human 1-D-myo-inositol-3-phosphate synthase is functional in yeast. (2004). https://pubmed.ncbi.nlm.nih.gov/15024000/ DOI: 10.1074/jbc.m312078200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f7adbcedad647d5f8a3cc9e46bfa515e83c508be9c2a4e9d59c5f5dfb0667d53","revision_id":"a82d187c-60fb-52d1-8482-eadb92860f33","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}