{"id":"045fdcb0-405f-59e1-846c-320ea6613bac","stable_key":"49fecf36-4958-5f15-8c78-9a68cba50763:capsaicin-binding-pose","predicate":"stabilizes_open_state_of","statement":"Capsaicin binds TRPV1 in a tail-up, head-down configuration and stabilizes the open state through vanillyl-group contact with the S4-S5 linker.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"1ca9eee1-098a-5fb4-a672-e8c4b7fa51fd","mechanism_event_label":"Capsaicin binds TRPV1 in a tail-up, head-down configuration and stabilizes the open state through vanillyl-group contact with the S4-S5 linker.","subject":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"object":{"id":"8e0c61d8-3930-51d5-bce2-25a5edfb797c","slug":"human-trpv1","display_name":"Human transient receptor potential vanilloid 1 / TRPV1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"1ca9eee1-098a-5fb4-a672-e8c4b7fa51fd","stable_key":"49fecf36-4958-5f15-8c78-9a68cba50763:capsaicin-binding-pose-event","event_type":"observed_relationship","label":"Capsaicin binds TRPV1 in a tail-up, head-down configuration and stabilizes the open state through vanillyl-group contact with the S4-S5 linker.","description":"Capsaicin binds TRPV1 in a tail-up, head-down configuration and stabilizes the open state through vanillyl-group contact with the S4-S5 linker.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8e0c61d8-3930-51d5-bce2-25a5edfb797c","slug":"human-trpv1","display_name":"Human transient receptor potential vanilloid 1 / TRPV1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Capsaicin-bound channel","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"TRPV1 structural model with site-specific functional tests","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The study resolves a binding mechanism but does not specify human dietary pharmacokinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Capsaicin chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"TRPV1 structural model with site-specific functional tests","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Capsaicin binds TRPV1 in a tail-up, head-down configuration and stabilizes the open state through vanillyl-group contact with the S4-S5 linker.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Structural mechanism underlying capsaicin binding and activation of the TRPV1 ion channel. (2015). https://pubmed.ncbi.nlm.nih.gov/26053297/ DOI: 10.1038/nchembio.1835","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Structural and in-vitro mutational analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Ligand binding and channel opening","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ea818dc7-8d37-5b51-9872-9fbe55d74558","evidence_kind":"source_excerpt","locator":"Lines 22-31","start_line":22,"end_line":31,"excerpt":"## capsaicin-binding-pose\nCapsaicin binds TRPV1 in a tail-up, head-down configuration and stabilizes the open state through vanillyl-group contact with the S4-S5 linker.\nModel/species: TRPV1 structural model with site-specific functional tests\nTissue/system: Ligand binding and channel opening\nExposure: Capsaicin-bound channel\nRoute: Structural and in-vitro mutational analysis\nDuration: Acute\nLimits: The study resolves a binding mechanism but does not specify human dietary pharmacokinetics.\nPrimary reference: Structural mechanism underlying capsaicin binding and activation of the TRPV1 ion channel. (2015). https://pubmed.ncbi.nlm.nih.gov/26053297/ DOI: 10.1038/nchembio.1835\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"TRPV1 structural model with site-specific functional tests","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"883c8afb-af48-5561-ae3b-00d4f3c26752","stable_key":"import-49fecf36-4958-5f15-8c78-9a68cba50763","title":"Capsaicin: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. 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