{"id":"045622a9-3d75-565d-a430-25c95d2ba479","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-beta-atp-inhibition","predicate":"inhibits","statement":"Free ATP and nonhydrolyzable ATP analogues inhibited beta-cell KATP channels without requiring phosphorylation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"0f1c6d0c-9add-5c13-a878-f8be19fd3de5","mechanism_event_label":"ATP can close the potassium channel through nucleotide regulation.","subject":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"object":{"id":"a4a6ce6c-ada1-5c56-8adf-d2bddf959383","slug":"beta-cell-katp-current","display_name":"Pancreatic beta-cell ATP-sensitive potassium current","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0f1c6d0c-9add-5c13-a878-f8be19fd3de5","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-beta-atp-inhibition-event","event_type":"biochemical_relationship","label":"ATP can close the potassium channel through nucleotide regulation.","description":"Free ATP and nonhydrolyzable ATP analogues inhibited beta-cell KATP channels without requiring phosphorylation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"24904faf-f4b4-5e5a-a2e6-3549fb7a2a2e","slug":"mg-atp","display_name":"Magnesium-ATP complex","entity_type_key":"chemical_species"},"role":"separately tested nucleotide species","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"conducted ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a4a6ce6c-ada1-5c56-8adf-d2bddf959383","slug":"beta-cell-katp-current","display_name":"Pancreatic beta-cell ATP-sensitive potassium current","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Free ATP must be distinguished from its magnesium complex.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat beta-cell inside-out patches.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Excised patches do not reproduce all intact-cell metabolic regulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rat","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"ATP can close the potassium channel through nucleotide regulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ashcroft-1989-magnesium] ATP-sensitive K+ channels in rat pancreatic beta-cells: modulation by ATP and Mg2+ ions (1989). https://pmc.ncbi.nlm.nih.gov/articles/PMC1189219/ DOI: 10.1113/jphysiol.1989.sp017765","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Pancreatic beta-cell membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0ac90fa3-d055-5094-a0ab-672c25e4c390","evidence_kind":"source_excerpt","locator":"Lines 786-796","start_line":786,"end_line":796,"excerpt":"### k-beta-atp-inhibition\nFree ATP and nonhydrolyzable ATP analogues inhibited beta-cell KATP channels without requiring phosphorylation.\nCondition category: normal\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: ATP can close the potassium channel through nucleotide regulation.\norganism: Rat\ntissue_or_cell_type: Pancreatic beta-cell membrane\nexperimental_model: Rat beta-cell inside-out patches.\nlimitations: Excised patches do not reproduce all intact-cell metabolic regulation.\ncross_nutrient: Free ATP must be distinguished from its magnesium complex.\n[ashcroft-1989-magnesium] ATP-sensitive K+ channels in rat pancreatic beta-cells: modulation by ATP and Mg2+ ions (1989). https://pmc.ncbi.nlm.nih.gov/articles/PMC1189219/ DOI: 10.1113/jphysiol.1989.sp017765","model_system":"Rat beta-cell inside-out patches.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ashcroft-1989-magnesium] ATP-sensitive K+ channels in rat pancreatic beta-cells: modulation by ATP and Mg2+ ions (1989). https://pmc.ncbi.nlm.nih.gov/articles/PMC1189219/ DOI: 10.1113/jphysiol.1989.sp017765","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}