{"id":"03c368f0-cbd9-5c61-943c-2b468fd8e907","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:glucose-analogue-cyanidin-3-glucoside","predicate":"decreases_in_recorded_experiment","statement":"The tested cyanidin glycoside reduced fluorescent glucose-analogue uptake in Caco-2 BBe1 cells at 50 uM.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"1b15d584-fc76-5c7e-bcff-f0e9c3a437ba","mechanism_event_label":"The tested cyanidin glycoside reduced fluorescent glucose-analogue uptake in Caco-2 BBe1 cells at 50 uM.","subject":{"id":"75149db0-02f7-5d32-8c7a-5c111d90d8ee","slug":"cyanidin-3-glucoside","display_name":"Cyanidin 3-O-beta-D-glucopyranoside","entity_type_key":"small_molecule"},"object":{"id":"0ccebf65-eac8-56ff-b770-0b39e9d7185d","slug":"human-caco2-2nbdg-uptake","display_name":"2-NBDG uptake by human Caco-2 BBe1 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1b15d584-fc76-5c7e-bcff-f0e9c3a437ba","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:glucose-analogue-cyanidin-3-glucoside-event","event_type":"experimental_observation","label":"The tested cyanidin glycoside reduced fluorescent glucose-analogue uptake in Caco-2 BBe1 cells at 50 uM.","description":"**Glucose handling is a separate cross-nutrient interaction.** A Caco-2 BBe1 study tested cyanidin and glycosides alongside quercetin compounds. At 50 µM, the glycosides reduced uptake of the fluorescent glucose analogue 2-NBDG. Transport inhibition and SGLT1 knockdown supported carrier involvement in glycoside uptake, with different behavior for aglycones. This is a nutrient-transport interaction in cultured cells; it is not proof that ordinary berry intake blocks intestinal glucose absorption or causes carbohydrate deficiency. [Zhang et al., 2020, transport and glucose-uptake experiments](https://pmc.ncbi.nlm.nih.gov/articles/PMC7240828/).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"75149db0-02f7-5d32-8c7a-5c111d90d8ee","slug":"cyanidin-3-glucoside","display_name":"Cyanidin 3-O-beta-D-glucopyranoside","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"50 uM cyanidin-3-glucoside","sequence_order":0,"notes":""},{"entity":{"id":"0ccebf65-eac8-56ff-b770-0b39e9d7185d","slug":"human-caco2-2nbdg-uptake","display_name":"2-NBDG uptake by human Caco-2 BBe1 cells","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"7a42c08c-7a63-5d46-990f-934b3f2bbbe0","slug":"2-nbdg","display_name":"2-NBDG, fluorescent glucose-uptake analogue","entity_type_key":"small_molecule"},"role":"measured glucose analogue","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"nutrient represented by the analogue","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"81db77cc-b909-593a-923e-4e52841491aa","slug":"slc5a1","display_name":"Human sodium/glucose cotransporter SGLT1 / SLC5A1","entity_type_key":"protein"},"role":"investigated transporter","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"5a760e20-e07a-5725-8f72-8d4b64e71d6b","slug":"slc2a2","display_name":"Human glucose transporter 2 / SLC2A2 / GLUT2","entity_type_key":"protein"},"role":"investigated transporter","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Full text retrieved; relevant methods/results/figure text reviewed. No independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"50 uM cyanidin-3-glucoside\", \"comparator\": \"Cell assay without added glycoside\", \"endpoint\": \"The tested cyanidin glycoside reduced fluorescent glucose-analogue uptake in Caco-2 BBe1 cells at 50 uM.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Human Caco-2 BBe1; 50 uM flavonoid pretreatment, fluorescent 2-NBDG uptake; not dietary human glucose absorption.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Fluorescent analogue outcome, not verified clinical glycemic benefit or carbohydrate deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The tested cyanidin glycoside reduced fluorescent glucose-analogue uptake in Caco-2 BBe1 cells at 50 uM.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Molecular Mechanisms Underlying the Absorption of Aglycone and Glycosidic Flavonoids in a Caco-2 BBe1 Cell Model. | 2020 | DOI 10.1021/acsomega.0c00379 | PMID 32455198 | https://pubmed.ncbi.nlm.nih.gov/32455198/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC7240828/ | https://doi.org/10.1021/acsomega.0c00379","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 40-40; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"22adcae5-8d99-5e9e-b7e5-8e7fb0c6b89f","evidence_kind":"source_excerpt","locator":"Lines 40-40","start_line":40,"end_line":40,"excerpt":"**Glucose handling is a separate cross-nutrient interaction.** A Caco-2 BBe1 study tested cyanidin and glycosides alongside quercetin compounds. At 50 µM, the glycosides reduced uptake of the fluorescent glucose analogue 2-NBDG. Transport inhibition and SGLT1 knockdown supported carrier involvement in glycoside uptake, with different behavior for aglycones. This is a nutrient-transport interaction in cultured cells; it is not proof that ordinary berry intake blocks intestinal glucose absorption or causes carbohydrate deficiency. [Zhang et al., 2020, transport and glucose-uptake experiments](https://pmc.ncbi.nlm.nih.gov/articles/PMC7240828/).","model_system":"Human Caco-2 BBe1; 50 uM flavonoid pretreatment, fluorescent 2-NBDG uptake; not dietary human glucose absorption.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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