{"id":"03c0f436-282b-59de-b4b7-efec46c85731","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:acute-blueberry-nox","predicate":"decreases_in_recorded_experiment","statement":"Acute blueberry polyphenol intake was associated with lower neutrophil NADPH oxidase activity at times of improved FMD.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"7484ec03-cfc1-5f1e-b71e-10556c1006e8","mechanism_event_label":"Acute blueberry polyphenol intake was associated with lower neutrophil NADPH oxidase activity at times of improved FMD.","subject":{"id":"8f12b9ac-46fe-5bef-bd17-cca7f22f1668","slug":"blueberry-polyphenol-drink","display_name":"Blueberry polyphenol drink in acute vascular studies","entity_type_key":"botanical"},"object":{"id":"cf1608a3-2ffe-559b-973a-8adce98ed2d1","slug":"human-neutrophil-nox-activity","display_name":"Human neutrophil NADPH oxidase activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7484ec03-cfc1-5f1e-b71e-10556c1006e8","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:acute-blueberry-nox-event","event_type":"experimental_observation","label":"Acute blueberry polyphenol intake was associated with lower neutrophil NADPH oxidase activity at times of improved FMD.","description":"**Blueberry FMD timing tracks a changing exposure.** Acute crossover experiments in healthy men found FMD increases at 1–2 and six hours, associated with circulating phenolics and lower neutrophil NADPH-oxidase activity. A plateau occurred above roughly 766 mg of total blueberry polyphenols in that protocol. This was neither 766 mg purified anthocyanins nor a universal optimum. Neutrophil measurements cannot be relabeled direct endothelial NOX inhibition. [Rodriguez-Mateos et al., 2013](https://pubmed.ncbi.nlm.nih.gov/24004888/).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8f12b9ac-46fe-5bef-bd17-cca7f22f1668","slug":"blueberry-polyphenol-drink","display_name":"Blueberry polyphenol drink in acute vascular studies","entity_type_key":"botanical"},"role":"tested factor","stoichiometry":null,"state_label":"Blueberry polyphenol drink","sequence_order":0,"notes":""},{"entity":{"id":"cf1608a3-2ffe-559b-973a-8adce98ed2d1","slug":"human-neutrophil-nox-activity","display_name":"Human neutrophil NADPH oxidase activity","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"a557fb69-ee27-5be9-8318-227c570267ff","slug":"flow-mediated-dilation","display_name":"Brachial artery flow-mediated dilation","entity_type_key":"cellular_process"},"role":"correlated vascular endpoint","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full methods, figures, exact doses or endpoint-specific species assignments may remain unextracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Blueberry polyphenol drink\", \"comparator\": \"Matched control drink\", \"endpoint\": \"Acute blueberry polyphenol intake was associated with lower neutrophil NADPH oxidase activity at times of improved FMD.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Healthy-men crossover studies; 1-2 and 6-hour sampling; measurements in neutrophils.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Neutrophil assay and temporal association; not direct endothelial NOX binding or proof of mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Acute blueberry polyphenol intake was associated with lower neutrophil NADPH oxidase activity at times of improved FMD.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Intake and time dependence of blueberry flavonoid-induced improvements in vascular function: a randomized, controlled, double-blind, crossover intervention study with mechanistic insights into biological activity. | 2013 | DOI 10.3945/ajcn.113.066639 | PMID 24004888 | https://pubmed.ncbi.nlm.nih.gov/24004888/ | https://doi.org/10.3945/ajcn.113.066639","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 54-54; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a8750f6f-2c7b-5d52-89fa-ecb98a798c4f","evidence_kind":"source_excerpt","locator":"Lines 54-54","start_line":54,"end_line":54,"excerpt":"**Blueberry FMD timing tracks a changing exposure.** Acute crossover experiments in healthy men found FMD increases at 1–2 and six hours, associated with circulating phenolics and lower neutrophil NADPH-oxidase activity. A plateau occurred above roughly 766 mg of total blueberry polyphenols in that protocol. This was neither 766 mg purified anthocyanins nor a universal optimum. Neutrophil measurements cannot be relabeled direct endothelial NOX inhibition. [Rodriguez-Mateos et al., 2013](https://pubmed.ncbi.nlm.nih.gov/24004888/).","model_system":"Healthy-men crossover studies; 1-2 and 6-hour sampling; measurements in neutrophils.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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