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(2020). https://pubmed.ncbi.nlm.nih.gov/32214246/ DOI: 10.1038/s41586-020-2101-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Oral gavage and experimental microbiota/gene perturbation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Portal acetate and hepatic fatty-acid labeling","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"18b528c8-3284-51dc-a602-8a852a775fe2","evidence_kind":"source_excerpt","locator":"Lines 221-231","start_line":221,"end_line":231,"excerpt":"## hfcs-acss2-silencing\nSilencing hepatic Acss2 suppressed conversion of bolus fructose carbon into hepatic acetyl-CoA and fatty acids in mice.\nModel/species: Wild-type mice, microbiota depletion and liver Acss2-silencing experiments\nTissue: Portal acetate and hepatic fatty-acid labeling\nExposure: Bolus 2 g/kg labeled fructose plus 2 g/kg glucose; interventions specified by claim\nRoute: Oral gavage and experimental microbiota/gene perturbation\nDuration: Acute isotope sampling up to 6 h\nExposure scope: Component mixture\nLimits: Carbon tracing supports microbial acetate supply; antibiotic depletion is not a clinical recommendation and microbiota effects are not universal across diets.\nReference: Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate. 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