{"id":"03a0a058-980e-5a5a-aade-736aab2c0117","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-coq3-magnesium","predicate":"increases_assay_activity_of","statement":"Added magnesium increased reconstructed COQ3 activity about fourfold and improved substrate binding; EDTA abolished turnover.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cd8a5c8a-444f-50d9-9ecb-7d90c17cda0a","mechanism_event_label":"Magnesium changed a specific methylation step in this biochemical system.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"1ef33c17-ea2a-5690-a325-711e93d0da58","slug":"tet-coq3","display_name":"Reconstructed ancestral tetrapod COQ3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"cd8a5c8a-444f-50d9-9ecb-7d90c17cda0a","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-coq3-magnesium-event","event_type":"biochemical_relationship","label":"Magnesium changed a specific methylation step in this biochemical system.","description":"Added magnesium increased reconstructed COQ3 activity about fourfold and improved substrate binding; EDTA abolished turnover.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1ef33c17-ea2a-5690-a325-711e93d0da58","slug":"tet-coq3","display_name":"Reconstructed ancestral tetrapod COQ3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coq10-research/38425362.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d25af4ecd7340649536b8fea3b8a0a308a1611bbf8c57f6a79b362f443f1cba\", \"start_char\": 13814, \"end_char\": 15577, \"text_sha256\": \"c43de1ac5dc6711fad8e37af3a52a8cd9784ed7eeaf77bd2981afe72abaf307f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified reconstructed COQ metabolon with short-chain substrates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Enzyme combinations, methyl donors, reductants and metal additions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ancestral proteins and CoQ1 analogues; no clinical cofactor dose or proof of nutritional rate limitation. Reaction order need not be universal across species.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coq10","display_name":"Coenzyme Q10 / CoQ10 redox system","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Reconstructed ancestral tetrapod proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Magnesium changed a specific methylation step in this biochemical system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Stepwise CoQ head-group assembly","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"01badb18-8e42-5322-a5ff-806d0c76034d","evidence_kind":"source_excerpt","locator":"Lines 658-669","start_line":658,"end_line":669,"excerpt":"### coq10-coq3-magnesium\nAdded magnesium increased reconstructed COQ3 activity about fourfold and improved substrate binding; EDTA abolished turnover.\nCondition category: normal\nnutrient_topic: Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Magnesium changed a specific methylation step in this biochemical system.\norganism: Reconstructed ancestral tetrapod proteins\ntissue_or_cell_type: Stepwise CoQ head-group assembly\nexperimental_model: Purified reconstructed COQ metabolon with short-chain substrates\nlimitations: Ancestral proteins and CoQ1 analogues; no clinical cofactor dose or proof of nutritional rate limitation. Reaction order need not be universal across species.\nexposure: Enzyme combinations, methyl donors, reductants and metal additions\nevidence_span: {\"source_cache\": \"artifacts/coq10-research/38425362.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d25af4ecd7340649536b8fea3b8a0a308a1611bbf8c57f6a79b362f443f1cba\", \"start_char\": 13814, \"end_char\": 15577, \"text_sha256\": \"c43de1ac5dc6711fad8e37af3a52a8cd9784ed7eeaf77bd2981afe72abaf307f\"}\n[coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","model_system":"Purified reconstructed COQ metabolon with short-chain substrates","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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