{"id":"038a65ec-0d7b-5984-adb7-8eed47ed4981","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:thiamine-def-regional-energy-loss","predicate":"antagonist-assisted-depletion-reduces","statement":"Before neurological signs, total high-energy phosphates were 89% of control in diencephalon and 91% in lower brainstem; after signs, 76% and 79%.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"2d5f55f2-7ab1-5298-bd15-5fe2203aed27","mechanism_event_label":"Energy reserves fell early in two vulnerable brain regions.","subject":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"object":{"id":"241f94dc-56ca-58d4-9b4f-8a18c4d1806f","slug":"brain-high-energy-phosphate-content","display_name":"Brain high-energy phosphate content","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2d5f55f2-7ab1-5298-bd15-5fe2203aed27","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:thiamine-def-regional-energy-loss-event","event_type":"biochemical_relationship","label":"Energy reserves fell early in two vulnerable brain regions.","description":"Before neurological signs, total high-energy phosphates were 89% of control in diencephalon and 91% in lower brainstem; after signs, 76% and 79%.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"measured energy phosphate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c081984a-e118-582f-ac46-6a767f8f0bf2","slug":"phosphocreatine","display_name":"Phosphocreatine","entity_type_key":"small_molecule"},"role":"measured energy reserve","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"75a7f374-5ae9-5565-9f52-388e627bb492","slug":"pyrithiamine","display_name":"Pyrithiamine","entity_type_key":"small_molecule"},"role":"experimental antagonist","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"241f94dc-56ca-58d4-9b4f-8a18c4d1806f","slug":"brain-high-energy-phosphate-content","display_name":"Brain high-energy phosphate content","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_location","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"before the onset of neurological signs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Adult male Wistar rats with pyrithiamine-induced acute thiamine-deficient encephalopathy; microwave fixation and regional ATP/phosphocreatine assays before and after neurological signs.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Pyrithiamine-induced severe thiamine deficiency in rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Combined thiamine withdrawal and pyrithiamine exposure; not an estimate for mild dietary insufficiency or a human blood threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Thiamine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Energy reserves fell early in two vulnerable brain regions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[aikawa-1984-brain-energy] Low energy levels in thiamine-deficient encephalopathy. (1984). https://pubmed.ncbi.nlm.nih.gov/6726285/ DOI: 10.1097/00005072-198405000-00006","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Diencephalon and lower brainstem","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2bd30c05-bd7b-5ad6-a884-ed4d5e1ea2ef","evidence_kind":"source_excerpt","locator":"Lines 1070-1082","start_line":1070,"end_line":1082,"excerpt":"### thiamine-def-regional-energy-loss\nBefore neurological signs, total high-energy phosphates were 89% of control in diencephalon and 91% in lower brainstem; after signs, 76% and 79%.\nCondition category: nutrient_deficiency\nnutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Energy reserves fell early in two vulnerable brain regions.\norganism: Rattus norvegicus\ntissue_or_cell_type: Diencephalon and lower brainstem\nexperimental_model: Adult male Wistar rats with pyrithiamine-induced acute thiamine-deficient encephalopathy; microwave fixation and regional ATP/phosphocreatine assays before and after neurological signs.\nlimitations: Combined thiamine withdrawal and pyrithiamine exposure; not an estimate for mild dietary insufficiency or a human blood threshold.\nevidence_location: Abstract\nevidence_span: before the onset of neurological signs\nexposure: Pyrithiamine-induced severe thiamine deficiency in rats\n[aikawa-1984-brain-energy] Low energy levels in thiamine-deficient encephalopathy. (1984). https://pubmed.ncbi.nlm.nih.gov/6726285/ DOI: 10.1097/00005072-198405000-00006","model_system":"Adult male Wistar rats with pyrithiamine-induced acute thiamine-deficient encephalopathy; microwave fixation and regional ATP/phosphocreatine assays before and after neurological signs.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [aikawa-1984-brain-energy] Low energy levels in thiamine-deficient encephalopathy. 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