{"id":"0362931e-b2b1-55fa-9666-92b8707fe962","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-fdxr-fdx2","predicate":"transfers_electrons_to","statement":"FDX2 accepted electrons from reduced FDXR in the reconstructed system.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d9816644-4298-5b45-9760-031d68f32057","mechanism_event_label":"The synthesis pathway has a separate relay supplying reducing power.","subject":{"id":"a326b937-6a2f-52b0-9825-d79adbc4b2fe","slug":"tet-fdxr","display_name":"Reconstructed ancestral tetrapod FDXR","entity_type_key":"protein"},"object":{"id":"0c81d159-49cc-5be8-996e-7cee8944d794","slug":"tet-fdx2","display_name":"Reconstructed ancestral tetrapod FDX2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d9816644-4298-5b45-9760-031d68f32057","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-fdxr-fdx2-event","event_type":"biochemical_relationship","label":"The synthesis pathway has a separate relay supplying reducing power.","description":"FDX2 accepted electrons from reduced FDXR in the reconstructed system.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"physiological_reductant_in_coupled_assay","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a326b937-6a2f-52b0-9825-d79adbc4b2fe","slug":"tet-fdxr","display_name":"Reconstructed ancestral tetrapod FDXR","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0c81d159-49cc-5be8-996e-7cee8944d794","slug":"tet-fdx2","display_name":"Reconstructed ancestral tetrapod FDX2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coq10-research/38425362.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d25af4ecd7340649536b8fea3b8a0a308a1611bbf8c57f6a79b362f443f1cba\", \"start_char\": 9067, \"end_char\": 13813, \"text_sha256\": \"49612ae4d3a59a75a02fe3fb132abd8aeb7ef8b7cae3b0f79b5557503c55ad51\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified reconstructed COQ metabolon with short-chain substrates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Enzyme combinations, methyl donors, reductants and metal additions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ancestral proteins and CoQ1 analogues; no clinical cofactor dose or proof of nutritional rate limitation. Reaction order need not be universal across species.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coq10","display_name":"Coenzyme Q10 / CoQ10 redox system","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Reconstructed ancestral tetrapod proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The synthesis pathway has a separate relay supplying reducing power.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Stepwise CoQ head-group assembly","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1912b2b8-c2ac-5809-9a7c-e139945ef2a7","evidence_kind":"source_excerpt","locator":"Lines 619-630","start_line":619,"end_line":630,"excerpt":"### coq10-fdxr-fdx2\nFDX2 accepted electrons from reduced FDXR in the reconstructed system.\nCondition category: normal\nnutrient_topic: Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The synthesis pathway has a separate relay supplying reducing power.\norganism: Reconstructed ancestral tetrapod proteins\ntissue_or_cell_type: Stepwise CoQ head-group assembly\nexperimental_model: Purified reconstructed COQ metabolon with short-chain substrates\nlimitations: Ancestral proteins and CoQ1 analogues; no clinical cofactor dose or proof of nutritional rate limitation. Reaction order need not be universal across species.\nexposure: Enzyme combinations, methyl donors, reductants and metal additions\nevidence_span: {\"source_cache\": \"artifacts/coq10-research/38425362.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d25af4ecd7340649536b8fea3b8a0a308a1611bbf8c57f6a79b362f443f1cba\", \"start_char\": 9067, \"end_char\": 13813, \"text_sha256\": \"49612ae4d3a59a75a02fe3fb132abd8aeb7ef8b7cae3b0f79b5557503c55ad51\"}\n[coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","model_system":"Purified reconstructed COQ metabolon with short-chain substrates","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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