{"id":"0354b5f9-dda7-5ec9-a8fa-c055e5b2140b","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-lat1-transport","predicate":"transports","statement":"Human LAT1 mediated theanine uptake in stably transfected S2 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"00203712-ae1d-56f6-896f-328f171532e0","mechanism_event_label":"The same amino acid has more than one identified transport route.","subject":{"id":"16d078ff-692c-50eb-a34b-c61ef43821e1","slug":"slc7a5","display_name":"Human L-type amino acid transporter 1 / LAT1 / SLC7A5","entity_type_key":"protein"},"object":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"00203712-ae1d-56f6-896f-328f171532e0","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-lat1-transport-event","event_type":"biochemical_relationship","label":"The same amino acid has more than one identified transport route.","description":"Human LAT1 mediated theanine uptake in stably transfected S2 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"16d078ff-692c-50eb-a34b-c61ef43821e1","slug":"slc7a5","display_name":"Human L-type amino acid transporter 1 / LAT1 / SLC7A5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/theanine-research/23221699.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0f79abd668759eccd12f86ec895af75dadf94f6412c29d51a2b6cfde712f9372\", \"start_char\": 0, \"end_char\": 1143, \"text_sha256\": \"0f79abd668759eccd12f86ec895af75dadf94f6412c29d51a2b6cfde712f9372\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell uptake and stable human-transporter expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Radiolabeled theanine with leucine/BCH competition and sodium replacement","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human transporter identity confirmed in the public primary PDF methods. Engineered cells do not prove which transporter dominates human blood-brain-barrier flux or a clinical meal interaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Theanine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mammalian cell lines; human LAT1 and LAT2 expressed in mouse S2 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The same amino acid has more than one identified transport route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[theanine-p23221699] The involvement of L-type amino acid transporters in theanine transport. (2012). https://pubmed.ncbi.nlm.nih.gov/23221699/ DOI: 10.1271/bbb.120519","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"System L amino acid transport","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Recorded as theanine uptake in stably transfected S2 cells.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Recorded as theanine uptake in stably transfected S2 cells.","entity":null}],"evidence":[{"id":"704d4bc7-3df9-59da-b525-ace7c4d6c97d","evidence_kind":"source_excerpt","locator":"Lines 172-183","start_line":172,"end_line":183,"excerpt":"### theanine-lat1-transport\nHuman LAT1 mediated theanine uptake in stably transfected S2 cells.\nCondition category: normal\nnutrient_topic: L-Theanine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The same amino acid has more than one identified transport route.\norganism: Mammalian cell lines; human LAT1 and LAT2 expressed in mouse S2 cells\ntissue_or_cell_type: System L amino acid transport\nexperimental_model: Cell uptake and stable human-transporter expression\nlimitations: Human transporter identity confirmed in the public primary PDF methods. Engineered cells do not prove which transporter dominates human blood-brain-barrier flux or a clinical meal interaction.\nexposure: Radiolabeled theanine with leucine/BCH competition and sodium replacement\nevidence_span: {\"source_cache\": \"artifacts/theanine-research/23221699.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0f79abd668759eccd12f86ec895af75dadf94f6412c29d51a2b6cfde712f9372\", \"start_char\": 0, \"end_char\": 1143, \"text_sha256\": \"0f79abd668759eccd12f86ec895af75dadf94f6412c29d51a2b6cfde712f9372\"}\n[theanine-p23221699] The involvement of L-type amino acid transporters in theanine transport. (2012). https://pubmed.ncbi.nlm.nih.gov/23221699/ DOI: 10.1271/bbb.120519","model_system":"Cell uptake and stable human-transporter expression","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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