{"id":"0336ef97-b513-5f5a-9c01-f3092a76ce63","stable_key":"e37461ea-ea5d-5e2c-8091-138305f6dd70:l-aspartate-shuttle-aspartate-availability","predicate":"increases_shuttle_engagement","statement":"Increasing aspartate availability enhanced malate–aspartate shuttle use and mitochondrial metabolism of glucose-derived pyruvate in proliferating cell experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e0562cc5-467e-5655-985c-336e82724ff5","mechanism_event_label":"Aspartate demand for building material can compete with its participation in redox transfer.","subject":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"object":{"id":"d10d7b3b-32bd-5150-aa4b-b3b84aefb7e2","slug":"human-cell-malate-aspartate-shuttle-flux","display_name":"Malate–aspartate shuttle use in human proliferating cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e0562cc5-467e-5655-985c-336e82724ff5","stable_key":"e37461ea-ea5d-5e2c-8091-138305f6dd70:l-aspartate-shuttle-aspartate-availability-event","event_type":"observed_relationship","label":"Aspartate demand for building material can compete with its participation in redox transfer.","description":"Increasing aspartate availability enhanced malate–aspartate shuttle use and mitochondrial metabolism of glucose-derived pyruvate in proliferating cell experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d10d7b3b-32bd-5150-aa4b-b3b84aefb7e2","slug":"human-cell-malate-aspartate-shuttle-flux","display_name":"Malate–aspartate shuttle use in human proliferating cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4c4eee53-67f4-5c46-aa5f-ba2bcaf3424d","slug":"got1","display_name":"Human cytosolic aspartate aminotransferase / GOT1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c52a2a79-ca29-5b9c-b8bb-f8481d407841","slug":"got2","display_name":"Human mitochondrial aspartate aminotransferase / GOT2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"91e6d4f5-fba1-542d-b68a-57cc28e1e425","slug":"pyruvate","display_name":"Pyruvate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":7,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract and primary figure descriptions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Proliferating-cell perturbation and tracer study, including human NSCLC models.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Substrate availability in cell models is not evidence for benefits of oral L-aspartate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Aspartate collection; 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