{"id":"02ea5b4e-c157-5f0c-b420-7e6c8b9dd39c","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-pycr3-reduction","predicate":"reduces_p5c_to","statement":"Human PYCRL/PYCR3 localized predominantly to the cytosol in the tested melanoma cells and was more efficient with NADPH in the biochemical comparison.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2c76e79d-62fe-5d13-a54b-9ba1020153d0","mechanism_event_label":"The cytosolic version has different preferences from the mitochondrial enzymes.","subject":{"id":"6f3233f1-60ce-5c1d-ac42-394ceae60c5e","slug":"pycr3","display_name":"Human pyrroline-5-carboxylate reductase 3 / PYCR3 / PYCRL","entity_type_key":"protein"},"object":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"2c76e79d-62fe-5d13-a54b-9ba1020153d0","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-pycr3-reduction-event","event_type":"observed_relationship","label":"The cytosolic version has different preferences from the mitochondrial enzymes.","description":"Human PYCRL/PYCR3 localized predominantly to the cytosol in the tested melanoma cells and was more efficient with NADPH in the biochemical comparison.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f3233f1-60ce-5c1d-ac42-394ceae60c5e","slug":"pycr3","display_name":"Human pyrroline-5-carboxylate reductase 3 / PYCR3 / PYCRL","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"cbf8b66e-3d66-53f0-9c21-bc9c014131e7","slug":"pyrroline-5-carboxylate","display_name":"Delta-1-pyrroline-5-carboxylate / P5C","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"be6e4670-953f-5a4e-97da-1e536a54e0e4","slug":"nadp-plus","display_name":"NADP+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Lu1205 cell fractionation and recombinant human PYCRL kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The melanoma routing pattern is not asserted to apply identically in every human tissue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The cytosolic version has different preferences from the mitochondrial enzymes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Functional specialization in proline biosynthesis of melanoma. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23024808/ · DOI 10.1371/journal.pone.0045190","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8813c0f3-49ee-55fd-9fdb-5095f581f7e6","evidence_kind":"source_excerpt","locator":"Lines 30-36","start_line":30,"end_line":36,"excerpt":"## l-proline-pycr3-reduction\nThe cytosolic version has different preferences from the mitochondrial enzymes.\nHuman PYCRL/PYCR3 localized predominantly to the cytosol in the tested melanoma cells and was more efficient with NADPH in the biochemical comparison.\nModel: Lu1205 cell fractionation and recombinant human PYCRL kinetics.\nLimitations: The melanoma routing pattern is not asserted to apply identically in every human tissue.\nEvidence access: Primary full text\nFunctional specialization in proline biosynthesis of melanoma. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23024808/ · DOI 10.1371/journal.pone.0045190","model_system":"Lu1205 cell fractionation and recombinant human PYCRL kinetics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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