{"id":"02e69b25-831b-561b-9c9c-583f7ca86750","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:rat-tsa-platelet-camp","predicate":"increases_in_recorded_experiment","statement":"TSA showed the recorded increase in Rat TSA-associated platelet cAMP.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"f6ba914b-ea2c-5c4a-ad2e-ef805fa53257","mechanism_event_label":"TSA showed the recorded increase in Rat TSA-associated platelet cAMP.","subject":{"id":"a9913acc-3d53-539e-8d43-78cd5f7590ad","slug":"triacetylshikimic-acid","display_name":"Triacetylshikimic acid / TSA","entity_type_key":"small_molecule"},"object":{"id":"8c667bad-549a-5d8c-b442-67590cb2ae9d","slug":"rat-tsa-platelet-camp","display_name":"Rat TSA-associated platelet cAMP","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f6ba914b-ea2c-5c4a-ad2e-ef805fa53257","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:rat-tsa-platelet-camp-event","event_type":"experimental_observation","label":"TSA showed the recorded increase in Rat TSA-associated platelet cAMP.","description":"**Derivatives keep their own outcomes.** Triacetylshikimic acid (TSA) reduced stimulated platelet aggregation and shunt thrombosis in rat experiments; reported cAMP increased, while cGMP, thromboxane/prostacyclin metabolites and clotting-time endpoints included null results. Those results are not assigned to parent shikimic acid. The separately tested 3,4-O-isopropylidene derivative (ISA) has rodent inflammatory/pain studies and cell-free radical-scavenging assays. Structural similarity does not establish interchangeable pharmacokinetics, targets or safety. [Anti-platelet and anti-thrombotic effects of triacetylshikimic acid in rats.](https://pubmed.ncbi.nlm.nih.gov/11791012/) [Protective effects of 3,4-oxo-isopropylidene-shikimic acid on experimental colitis induced by trinitrobenzenesulfonic acid in rats.](https://pubmed.ncbi.nlm.nih.gov/22476587/) [Anti-inflammatory, analgesic and antioxidant activities of 3,4-oxo-isopropylidene-shikimic acid.](https://pubmed.ncbi.nlm.nih.gov/27609150/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a9913acc-3d53-539e-8d43-78cd5f7590ad","slug":"triacetylshikimic-acid","display_name":"Triacetylshikimic acid / TSA","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"8c667bad-549a-5d8c-b442-67590cb2ae9d","slug":"rat-tsa-platelet-camp","display_name":"Rat TSA-associated platelet cAMP","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full results, tables and supplements not independently extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Triacetylshikimic acid rat experiments; indexed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Derivative-specific result. Unextracted dose/time remain unspecified; no transfer to shikimic acid.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"TSA showed the recorded increase in Rat TSA-associated platelet cAMP.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anti-inflammatory, analgesic and antioxidant activities of 3,4-oxo-isopropylidene-shikimic acid. | 2016 | DOI 10.3109/13880209.2016.1153663 | PMID 27609150 | https://pubmed.ncbi.nlm.nih.gov/27609150/ | https://doi.org/10.3109/13880209.2016.1153663","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anti-platelet and anti-thrombotic effects of triacetylshikimic acid in rats. | 2002 | DOI 10.1097/00005344-200202000-00013 | PMID 11791012 | https://pubmed.ncbi.nlm.nih.gov/11791012/ | https://doi.org/10.1097/00005344-200202000-00013","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Protective effects of 3,4-oxo-isopropylidene-shikimic acid on experimental colitis induced by trinitrobenzenesulfonic acid in rats. | 2012 | DOI 10.1007/s10620-012-2155-y | PMID 22476587 | https://pubmed.ncbi.nlm.nih.gov/22476587/ | https://doi.org/10.1007/s10620-012-2155-y","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 57-57; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b53dd87b-641e-57cb-a969-14fdad94550c","evidence_kind":"source_excerpt","locator":"Lines 57-57","start_line":57,"end_line":57,"excerpt":"**Derivatives keep their own outcomes.** Triacetylshikimic acid (TSA) reduced stimulated platelet aggregation and shunt thrombosis in rat experiments; reported cAMP increased, while cGMP, thromboxane/prostacyclin metabolites and clotting-time endpoints included null results. Those results are not assigned to parent shikimic acid. The separately tested 3,4-O-isopropylidene derivative (ISA) has rodent inflammatory/pain studies and cell-free radical-scavenging assays. Structural similarity does not establish interchangeable pharmacokinetics, targets or safety. [Anti-platelet and anti-thrombotic effects of triacetylshikimic acid in rats.](https://pubmed.ncbi.nlm.nih.gov/11791012/) [Protective effects of 3,4-oxo-isopropylidene-shikimic acid on experimental colitis induced by trinitrobenzenesulfonic acid in rats.](https://pubmed.ncbi.nlm.nih.gov/22476587/) [Anti-inflammatory, analgesic and antioxidant activities of 3,4-oxo-isopropylidene-shikimic acid.](https://pubmed.ncbi.nlm.nih.gov/27609150/)","model_system":"Triacetylshikimic acid rat experiments; indexed abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e6ae59de-0369-5c2f-8262-57d91302671c","stable_key":"import-31b1baa4-4113-5541-b9e7-fe44a5253a07","title":"Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"95b1f9e9577661312d67f36e156d2e49326b207f296c1c0e801a5b007fd8e283","revision_id":"cd3237f1-131a-557d-84b5-7543259807b0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}