{"id":"02b638d4-9a23-5928-9f06-d410f3f27ecf","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-a39-pool","predicate":"reduces","statement":"SLC25A39 loss reduced mitochondrial GSH abundance.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3d5cd14f-9b7f-5309-a4dd-e0013b07ff0c","mechanism_event_label":"The affected compartment lost its supply.","subject":{"id":"f3806ff7-01fc-5499-9956-0df1a027d2ac","slug":"slc25a39-knockout-cells","display_name":"SLC25A39-knockout mammalian cells","entity_type_key":"protein_state"},"object":{"id":"5b684abd-53cd-5120-8187-d92f052efa03","slug":"mitochondrial-gsh-pool","display_name":"Mammalian mitochondrial reduced-glutathione pool","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3d5cd14f-9b7f-5309-a4dd-e0013b07ff0c","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-a39-pool-event","event_type":"biochemical_relationship","label":"The affected compartment lost its supply.","description":"SLC25A39 loss reduced mitochondrial GSH abundance.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f3806ff7-01fc-5499-9956-0df1a027d2ac","slug":"slc25a39-knockout-cells","display_name":"SLC25A39-knockout mammalian cells","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5b684abd-53cd-5120-8187-d92f052efa03","slug":"mitochondrial-gsh-pool","display_name":"Mammalian mitochondrial reduced-glutathione pool","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/glutathione-research/34707288.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7e6a64f24da95b93d7440f3424bb8a611f3fc962a5dd6344f5d0e11671e0bba5\", \"start_char\": 0, \"end_char\": 1450, \"text_sha256\": \"7e6a64f24da95b93d7440f3424bb8a611f3fc962a5dd6344f5d0e11671e0bba5\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Organelle metabolomics, genetics and targeted synthesis rescue","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"SLC25A39 loss, A39/A40 double loss and targeted GshF","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Mitochondrial versus total-cell pools differ; engineered synthesis rescue is not oral supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glutathione research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mammalian cells; separate mouse erythropoiesis experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The affected compartment lost its supply.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[glutathione-p34707288] SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells. (2021). https://pubmed.ncbi.nlm.nih.gov/34707288/ DOI: 10.1038/s41586-021-04025-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"dcc43f3a-f0cb-5dc6-bb5f-b9cebb591b2c","evidence_kind":"source_excerpt","locator":"Lines 463-474","start_line":463,"end_line":474,"excerpt":"### glutathione-a39-pool\nSLC25A39 loss reduced mitochondrial GSH abundance.\nCondition category: machinery_impairment\nnutrient_topic: Glutathione research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The affected compartment lost its supply.\norganism: Mammalian cells; separate mouse erythropoiesis experiments\ntissue_or_cell_type: Mitochondria\nexperimental_model: Organelle metabolomics, genetics and targeted synthesis rescue\nlimitations: Mitochondrial versus total-cell pools differ; engineered synthesis rescue is not oral supplementation.\nexposure: SLC25A39 loss, A39/A40 double loss and targeted GshF\nevidence_span: {\"source_cache\": \"artifacts/glutathione-research/34707288.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7e6a64f24da95b93d7440f3424bb8a611f3fc962a5dd6344f5d0e11671e0bba5\", \"start_char\": 0, \"end_char\": 1450, \"text_sha256\": \"7e6a64f24da95b93d7440f3424bb8a611f3fc962a5dd6344f5d0e11671e0bba5\"}\n[glutathione-p34707288] SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells. (2021). https://pubmed.ncbi.nlm.nih.gov/34707288/ DOI: 10.1038/s41586-021-04025-w","model_system":"Organelle metabolomics, genetics and targeted synthesis rescue","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [glutathione-p34707288] SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells. (2021). https://pubmed.ncbi.nlm.nih.gov/34707288/ DOI: 10.1038/s41586-021-04025-w","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9b7417fd-059d-5683-b452-71d1e3cf82d0","stable_key":"import-db0fc92e-b5ef-5667-a4c5-3ef257edbc9b","title":"Glutathione: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2a5740c4a7e770aafe883fea47909b18bf5e174f211749935130a50f9dea2753","revision_id":"a64016dd-4b72-5954-a72d-0e61de854073","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}