{"id":"02b53203-b42f-5d66-9bfc-ab10b9fee20c","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-glucose-feedback","predicate":"inhibits_product_formation_by","statement":"Adding 0.7 mM glucose partially inhibited sucrose hydrolysis in the human intestinal brush-border enzyme preparation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"f17a9110-c0f4-5774-a15d-15b448f6aaa0","mechanism_event_label":"Adding 0.7 mM glucose partially inhibited sucrose hydrolysis in the human intestinal brush-border enzyme preparation.","subject":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"object":{"id":"9ae84f85-5051-57a8-b962-eae212573609","slug":"si","display_name":"Human sucrase-isomaltase / SI","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f17a9110-c0f4-5774-a15d-15b448f6aaa0","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-glucose-feedback-event","event_type":"observed_relationship","label":"Adding 0.7 mM glucose partially inhibited sucrose hydrolysis in the human intestinal brush-border enzyme preparation.","description":"Adding 0.7 mM glucose partially inhibited sucrose hydrolysis in the human intestinal brush-border enzyme preparation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9ae84f85-5051-57a8-b962-eae212573609","slug":"si","display_name":"Human sucrase-isomaltase / SI","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"82558bd0-1a84-574a-a04c-10609c9c5dba","slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"75 mM sucrose for expressed-SI activity; 0.7 mM added glucose in brush-border kinetic experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"1 hour at 37 C for expressed-enzyme activity; kinetic series for product inhibition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Selected primary full-text sections inspected via indexed PMC page; local XML has abstract only.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human SI expressed in COS-1 cells; isolated human intestinal brush border","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_scope","value_text":"Human enzyme mechanism","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Glucose feedback was measured in an enzyme preparation, not demonstrated as a whole-body blood-glucose control mechanism. Selected full-text methods/results inspected; archived PubMed/PMC XML contains abstract only.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sucrose chapter; direct sucrose observations are distinguished from shared component metabolism.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human SI expressed in COS-1 cells; isolated human intestinal brush border","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Adding 0.7 mM glucose partially inhibited sucrose hydrolysis in the human intestinal brush-border enzyme preparation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Structure-function analysis of human sucrase-isomaltase identifies key residues required for catalytic activity. (2017). https://pubmed.ncbi.nlm.nih.gov/28522605/ DOI: 10.1074/jbc.M117.791939","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro enzyme/substrate incubation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Brush-border carbohydrate digestion","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7820e4d3-0fdc-54eb-9426-dea7fab6057c","evidence_kind":"source_excerpt","locator":"Lines 43-53","start_line":43,"end_line":53,"excerpt":"## sucrose-glucose-feedback\nAdding 0.7 mM glucose partially inhibited sucrose hydrolysis in the human intestinal brush-border enzyme preparation.\nModel/species: Human SI expressed in COS-1 cells; isolated human intestinal brush border\nTissue: Brush-border carbohydrate digestion\nExposure: 75 mM sucrose for expressed-SI activity; 0.7 mM added glucose in brush-border kinetic experiments\nRoute: In vitro enzyme/substrate incubation\nDuration: 1 hour at 37 C for expressed-enzyme activity; kinetic series for product inhibition\nExposure scope: Human enzyme mechanism\nLimits: Glucose feedback was measured in an enzyme preparation, not demonstrated as a whole-body blood-glucose control mechanism. Selected full-text methods/results inspected; archived PubMed/PMC XML contains abstract only.\nReference: Structure-function analysis of human sucrase-isomaltase identifies key residues required for catalytic activity. (2017). https://pubmed.ncbi.nlm.nih.gov/28522605/ DOI: 10.1074/jbc.M117.791939\nAccess: Selected primary full-text sections inspected via indexed PMC page; local XML has abstract only.","model_system":"Human SI expressed in COS-1 cells; isolated human intestinal brush border","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; provenance retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"8e24ddf4-84ee-57b4-9fd8-093547432ece","stable_key":"import-c932089b-ebc9-5f39-b2e5-84af74f9f6d0","title":"Sucrose: mechanism of action and metabolic impact (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific sucrose curation with shared canonical claims retained by identity. Primary-study citations, negative findings, exposure details and limitations preserved. Not publisher full text.","file_path":"","sha256":"5e68ccbade7a80fc03778495756f0eeeeba5038cd10d5bef8e33cb1e93e4b044","revision_id":"6edb8ffc-11c5-5cd9-852c-85adb71165a8","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}