{"id":"02ab2c48-97a1-51d3-9dad-3bf8d70554dd","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vdm-calcitriol-induces-human-osteocalcin-transgene","predicate":"increases_expression","statement":"Calcitriol increased human osteocalcin mRNA about 1.8-fold in calvaria of mice carrying the human osteocalcin genomic transgene.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"3c1c0ff8-0be7-5656-925a-b02d147e378c","mechanism_event_label":"The human osteocalcin gene responded to active vitamin D in this engineered mouse model.","subject":{"id":"f5156c24-108f-5746-b114-ed764e79a3f4","slug":"calcitriol","display_name":"Calcitriol","entity_type_key":"small_molecule"},"object":{"id":"d52bb59a-1bf6-58d4-8d75-09946b40f428","slug":"human-bglap-mrna","display_name":"Human BGLAP mRNA","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"3c1c0ff8-0be7-5656-925a-b02d147e378c","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vdm-calcitriol-induces-human-osteocalcin-transgene-event","event_type":"biochemical_relationship","label":"The human osteocalcin gene responded to active vitamin D in this engineered mouse model.","description":"Calcitriol increased human osteocalcin mRNA about 1.8-fold in calvaria of mice carrying the human osteocalcin genomic transgene.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f5156c24-108f-5746-b114-ed764e79a3f4","slug":"calcitriol","display_name":"Calcitriol","entity_type_key":"small_molecule"},"role":"hormonal-regulator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b043ce5c-8537-5099-8421-b07ec993dbde","slug":"bglap","display_name":"Osteocalcin / BGLAP","entity_type_key":"protein"},"role":"encoded-protein","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d52bb59a-1bf6-58d4-8d75-09946b40f428","slug":"human-bglap-mrna","display_name":"Human BGLAP mRNA","entity_type_key":"rna"},"role":"measured-transcript","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Vitamin D–vitamin K boundary: vitamin D-regulated osteocalcin expression is distinct from subsequent vitamin K-dependent carboxylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_locator","value_text":"Abstract: reported experimental results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"D3 active metabolite or VDR machinery experiment; not a direct D2-versus-D3 comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human osteocalcin genomic transgene in mice; calvarial mRNA analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Repeated calcitriol administration; exact dose and calvarial mRNA collection time not specified in the retrieved abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human transgene in mouse tissue is not a human supplementation trial. Transcript induction does not measure vitamin K-dependent carboxylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin D2 and D3","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus with human BGLAP transgene","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The human osteocalcin gene responded to active vitamin D in this engineered mouse model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[vdm-zhang1997] Analysis of osteocalcin expression in transgenic mice reveals a species difference in vitamin D regulation of mouse and human osteocalcin genes. (1997). https://pubmed.ncbi.nlm.nih.gov/9333117/ DOI: 10.1359/jbmr.1997.12.10.1570","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Calvaria","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a53b451f-9cd1-54f8-b93b-7f141e0774f7","evidence_kind":"source_excerpt","locator":"Lines 857-871","start_line":857,"end_line":871,"excerpt":"### vdm-calcitriol-induces-human-osteocalcin-transgene\nCalcitriol increased human osteocalcin mRNA about 1.8-fold in calvaria of mice carrying the human osteocalcin genomic transgene.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The human osteocalcin gene responded to active vitamin D in this engineered mouse model.\norganism: Mus musculus with human BGLAP transgene\ntissue_or_cell_type: Calvaria\nexperimental_model: Human osteocalcin genomic transgene in mice; calvarial mRNA analysis\nlimitations: Human transgene in mouse tissue is not a human supplementation trial. Transcript induction does not measure vitamin K-dependent carboxylation.\nexposure: Repeated calcitriol administration; exact dose and calvarial mRNA collection time not specified in the retrieved abstract.\ncross_nutrient: Vitamin D–vitamin K boundary: vitamin D-regulated osteocalcin expression is distinct from subsequent vitamin K-dependent carboxylation.\nevidence_locator: Abstract: reported experimental results\nnutrient: Vitamin D2 and D3\nevidence_scope: D3 active metabolite or VDR machinery experiment; not a direct D2-versus-D3 comparison.\n[vdm-zhang1997] Analysis of osteocalcin expression in transgenic mice reveals a species difference in vitamin D regulation of mouse and human osteocalcin genes. (1997). https://pubmed.ncbi.nlm.nih.gov/9333117/ DOI: 10.1359/jbmr.1997.12.10.1570","model_system":"Human osteocalcin genomic transgene in mice; calvarial mRNA analysis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [vdm-zhang1997] Analysis of osteocalcin expression in transgenic mice reveals a species difference in vitamin D regulation of mouse and human osteocalcin genes. (1997). https://pubmed.ncbi.nlm.nih.gov/9333117/ DOI: 10.1359/jbmr.1997.12.10.1570","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c95c763c-5b31-5b3f-a2f8-2dfe5ef0d3ca","stable_key":"import-1deb434a-3547-51a0-a038-87b1ce79ec38","title":"Vitamin D2 and D3: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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