{"id":"02a5e745-ecbf-559b-8256-de5d7023ccb0","stable_key":"05a3cf47-e660-5ab3-8c32-5eba9847cf0a:thc-dagla-localisation","predicate":"is_localised_to","statement":"Diacylglycerol lipase-alpha was localised around the postsynaptic spine, placing the production site of 2-arachidonoylglycerol in close proximity to the presynaptic CB1 receptor.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"044666a3-262d-533e-85fd-52b3dc4b1fb4","mechanism_event_label":"The factory for the messenger sits directly across the synapse from its receptor.","subject":{"id":"db61034c-b170-5491-acb0-c955ce2e2251","slug":"dagla","display_name":"Diacylglycerol lipase alpha / DAGLA","entity_type_key":"protein"},"object":{"id":"7f9c5eaa-2a05-5756-90d7-bf02fee8499a","slug":"postsynaptic-spine","display_name":"The postsynaptic dendritic spine","entity_type_key":"cell_type"},"evidence_count":1,"mechanism_event":{"id":"044666a3-262d-533e-85fd-52b3dc4b1fb4","stable_key":"05a3cf47-e660-5ab3-8c32-5eba9847cf0a:thc-dagla-localisation-event","event_type":"biochemical_relationship","label":"The factory for the messenger sits directly across the synapse from its receptor.","description":"Diacylglycerol lipase-alpha was localised around the postsynaptic spine, placing the production site of 2-arachidonoylglycerol in close proximity to the presynaptic CB1 receptor.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e0aeb1b3-fbfb-5ac4-a29c-d5171072c256","slug":"2-arachidonoylglycerol","display_name":"2-Arachidonoylglycerol / 2-AG","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d092243c-94e9-5f03-a406-2fa245f2f4ae","slug":"cnr1","display_name":"Human cannabinoid receptor 1 / CB1 / CNR1","entity_type_key":"protein"},"role":"facing_receptor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"db61034c-b170-5491-acb0-c955ce2e2251","slug":"dagla","display_name":"Diacylglycerol lipase alpha / DAGLA","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7f9c5eaa-2a05-5756-90d7-bf02fee8499a","slug":"postsynaptic-spine","display_name":"The postsynaptic dendritic spine","entity_type_key":"cell_type"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/thc-research/16672646.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"dd4ddba9cf6987282758f17ce79c5624f9a3cc6c02044a4a45c0664ef6fd21c3\", \"start_char\": 0, \"end_char\": 1832, \"text_sha256\": \"dd4ddba9cf6987282758f17ce79c5624f9a3cc6c02044a4a45c0664ef6fd21c3\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Immunoelectron microscopy localising diacylglycerol lipase-alpha","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Subcellular localisation against presynaptic CB1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"An anatomical result that makes the signalling geometry concrete: the enzyme that makes the messenger sits directly opposite the receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"THC research collection; topical membership is not evidence of a direct clinical effect, and THC is recorded separately from the endocannabinoids it imitates.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tetrahydrocannabinol","display_name":"Delta-9-tetrahydrocannabinol / THC","entity_type_key":"drug"}},{"dimension":"organism","value_text":"Rodent","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The factory for the messenger sits directly across the synapse from its receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[thc-p16672646] Localization of diacylglycerol lipase-alpha around postsynaptic spine suggests close proximity between production site of an endocannabinoid, 2-arachidonoyl-glycerol, and presynaptic cannabinoid CB1 receptor. (2006). https://pubmed.ncbi.nlm.nih.gov/16672646/ DOI: 10.1523/jneurosci.0054-06.2006","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Postsynaptic spine","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"af11569a-6537-5fcb-9751-6875fbacfb17","evidence_kind":"source_excerpt","locator":"Lines 309-320","start_line":309,"end_line":320,"excerpt":"### thc-dagla-localisation\nDiacylglycerol lipase-alpha was localised around the postsynaptic spine, placing the production site of 2-arachidonoylglycerol in close proximity to the presynaptic CB1 receptor.\nCondition category: normal\nnutrient_topic: THC research collection; topical membership is not evidence of a direct clinical effect, and THC is recorded separately from the endocannabinoids it imitates.\nplain_language: The factory for the messenger sits directly across the synapse from its receptor.\norganism: Rodent\ntissue_or_cell_type: Postsynaptic spine\nexperimental_model: Immunoelectron microscopy localising diacylglycerol lipase-alpha\nlimitations: An anatomical result that makes the signalling geometry concrete: the enzyme that makes the messenger sits directly opposite the receptor.\nexposure: Subcellular localisation against presynaptic CB1\nevidence_span: {\"source_cache\": \"artifacts/thc-research/16672646.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"dd4ddba9cf6987282758f17ce79c5624f9a3cc6c02044a4a45c0664ef6fd21c3\", \"start_char\": 0, \"end_char\": 1832, \"text_sha256\": \"dd4ddba9cf6987282758f17ce79c5624f9a3cc6c02044a4a45c0664ef6fd21c3\"}\n[thc-p16672646] Localization of diacylglycerol lipase-alpha around postsynaptic spine suggests close proximity between production site of an endocannabinoid, 2-arachidonoyl-glycerol, and presynaptic cannabinoid CB1 receptor. (2006). https://pubmed.ncbi.nlm.nih.gov/16672646/ DOI: 10.1523/jneurosci.0054-06.2006","model_system":"Immunoelectron microscopy localising diacylglycerol lipase-alpha","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [thc-p16672646] Localization of diacylglycerol lipase-alpha around postsynaptic spine suggests close proximity between production site of an endocannabinoid, 2-arachidonoyl-glycerol, and presynaptic cannabinoid CB1 receptor. (2006). https://pubmed.ncbi.nlm.nih.gov/16672646/ DOI: 10.1523/jneurosci.0054-06.2006","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b4367320-9724-507a-bead-6932fa0a78cb","stable_key":"import-05a3cf47-e660-5ab3-8c32-5eba9847cf0a","title":"THC: the cannabinoid receptors, the endocannabinoid system it occupies, what the drug does, and the dietary fat it is built from (2026-09-21)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"60abc81737d18b7a50b01ca487e38291774ee15d9777651aa2d3eac8670ac3b2","revision_id":"2cba3552-af89-580d-b20a-8cfb1b14f9e7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}