{"id":"029d5f0b-9b32-54e3-8670-aad9afc729aa","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-brain-thtp-proton-motive-dependence","predicate":"supports","statement":"Depolarization or mitochondrial disruption inhibited ThTP synthesis in rat brain mitochondrial preparations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2183b0fa-67c3-5d07-93ac-09ea7f0ee72d","mechanism_event_label":"Intact, energized mitochondria were required for this B1-phosphate reaction.","subject":{"id":"2f9332e0-0138-5e42-8c7b-8f8f45615782","slug":"mitochondrial-membrane-potential","display_name":"Inner mitochondrial membrane potential","entity_type_key":"cellular_process"},"object":{"id":"68b2f05a-61b0-51e4-893e-63716c3a2d59","slug":"thiamine-triphosphate-synthesis","display_name":"Thiamine triphosphate synthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2183b0fa-67c3-5d07-93ac-09ea7f0ee72d","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-brain-thtp-proton-motive-dependence-event","event_type":"biochemical_relationship","label":"Intact, energized mitochondria were required for this B1-phosphate reaction.","description":"Depolarization or mitochondrial disruption inhibited ThTP synthesis in rat brain mitochondrial preparations.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"187db168-8028-5ce6-9f8b-4bc61ebad1a0","slug":"thiamine-diphosphate","display_name":"Thiamine diphosphate","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"baf0fa85-b008-55a1-b502-5adedf0382b8","slug":"thiamine-triphosphate","display_name":"Thiamine triphosphate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2f9332e0-0138-5e42-8c7b-8f8f45615782","slug":"mitochondrial-membrane-potential","display_name":"Inner mitochondrial membrane potential","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"68b2f05a-61b0-51e4-893e-63716c3a2d59","slug":"thiamine-triphosphate-synthesis","display_name":"Thiamine triphosphate synthesis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence-scope","value_text":"Brain mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_locator","value_text":"Results: ThTP synthesis in isolated mitochondria and energy coupling","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_spans","value_text":"[{\"source_document\": \"artifacts/thiamine_transport_sources/gangolf-2010-thtp-source-record.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1672}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated rat brain mitochondria; 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topical membership is not evidence of a direct dietary effect.\nplain_language: Intact, energized mitochondria were required for this B1-phosphate reaction.\norganism: Rattus norvegicus\ntissue_or_cell_type: Brain mitochondria\nexperimental_model: Isolated rat brain mitochondria; ThDP/Pi incubation, respiratory substrates and inhibitors.\nlimitations: Proton-motive-force dependence is supported; the proposed ATP-synthase identity was not definitively established.\nevidence_spans: [{\"source_document\": \"artifacts/thiamine_transport_sources/gangolf-2010-thtp-source-record.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1672}]\nevidence_locator: Results: ThTP synthesis in isolated mitochondria and energy coupling\nevidence-scope: Brain mitochondria\n[gangolf-2010-thtp] Thiamine Triphosphate Synthesis in Rat Brain Occurs in Mitochondria and Is Coupled to the Respiratory Chain (2010). https://pmc.ncbi.nlm.nih.gov/articles/PMC2804207/ DOI: 10.1074/jbc.M109.054379","model_system":"Isolated rat brain mitochondria; 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Study references: [gangolf-2010-thtp] Thiamine Triphosphate Synthesis in Rat Brain Occurs in Mitochondria and Is Coupled to the Respiratory Chain (2010). https://pmc.ncbi.nlm.nih.gov/articles/PMC2804207/ DOI: 10.1074/jbc.M109.054379","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"158d2c03-ac8c-589f-8270-c468165ae346","stable_key":"import-46d15d9e-d3b5-544d-ba01-b785aa3e4f42","title":"Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f376512fb3310141315548015b387833e3af45146e73fb73cd02cee20e4ddb9c","revision_id":"53bc5eda-dec8-58cc-a56f-a9eb4ab036ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}