{"id":"027d7a1d-05f7-596e-94fc-0714ee47e078","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-renal-membrane-potential","predicate":"drives","statement":"An inside-negative membrane potential drove uphill pantothenate accumulation in rabbit renal brush-border vesicles when sodium was present even without a sodium concentration gradient.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"95ab8365-e535-51b6-bdcf-3eac6a0a611f","mechanism_event_label":"Electrical potential also helps power renal pantothenate uptake.","subject":{"id":"0b20e447-c3ae-5f0d-ab07-536d781ad4aa","slug":"rabbit-renal-inside-negative-membrane-potential","display_name":"Inside-negative potential in rabbit renal brush-border vesicles","entity_type_key":"cellular_process"},"object":{"id":"3d57d1cd-3617-5b54-919b-0f82f1f27a8e","slug":"rabbit-renal-brush-border-pantothenate-uptake","display_name":"Rabbit renal brush-border pantothenate uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"95ab8365-e535-51b6-bdcf-3eac6a0a611f","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-renal-membrane-potential-event","event_type":"biochemical_relationship","label":"Electrical potential also helps power renal pantothenate uptake.","description":"An inside-negative membrane potential drove uphill pantothenate accumulation in rabbit renal brush-border vesicles when sodium was present even without a sodium concentration gradient.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"required_transport_ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0b20e447-c3ae-5f0d-ab07-536d781ad4aa","slug":"rabbit-renal-inside-negative-membrane-potential","display_name":"Inside-negative potential in rabbit renal brush-border vesicles","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"3d57d1cd-3617-5b54-919b-0f82f1f27a8e","slug":"rabbit-renal-brush-border-pantothenate-uptake","display_name":"Rabbit renal brush-border pantothenate uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated rabbit renal brush-border membrane vesicles with controlled ion gradients and membrane potentials","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Inside-negative membrane potential imposed with sodium present but no transmembrane sodium gradient.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rabbit isolated-membrane experiment; this predates molecular identification of SMVT and does not independently assign the effect to human SLC5A6 or establish a human renal clearance threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Oryctolagus cuniculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Electrical potential also helps power renal pantothenate uptake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-trans-renal1986] Pantothenate-sodium cotransport in renal brush-border membranes. (1986). https://pubmed.ncbi.nlm.nih.gov/3771539/ DOI: 10.1016/s0021-9258(18)66891-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Renal brush-border membrane vesicles","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6b3b55f2-4203-560f-91c0-994b03f42150","evidence_kind":"source_excerpt","locator":"Lines 249-260","start_line":249,"end_line":260,"excerpt":"### b5-trans-renal-membrane-potential\nAn inside-negative membrane potential drove uphill pantothenate accumulation in rabbit renal brush-border vesicles when sodium was present even without a sodium concentration gradient.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Electrical potential also helps power renal pantothenate uptake.\norganism: Oryctolagus cuniculus\ntissue_or_cell_type: Renal brush-border membrane vesicles\nexperimental_model: Isolated rabbit renal brush-border membrane vesicles with controlled ion gradients and membrane potentials\nlimitations: Rabbit isolated-membrane experiment; this predates molecular identification of SMVT and does not independently assign the effect to human SLC5A6 or establish a human renal clearance threshold.\nexposure: Inside-negative membrane potential imposed with sodium present but no transmembrane sodium gradient.\ncross_nutrient: true\n[b5-trans-renal1986] Pantothenate-sodium cotransport in renal brush-border membranes. (1986). https://pubmed.ncbi.nlm.nih.gov/3771539/ DOI: 10.1016/s0021-9258(18)66891-7","model_system":"Isolated rabbit renal brush-border membrane vesicles with controlled ion gradients and membrane potentials","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-trans-renal1986] Pantothenate-sodium cotransport in renal brush-border membranes. (1986). https://pubmed.ncbi.nlm.nih.gov/3771539/ DOI: 10.1016/s0021-9258(18)66891-7","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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