{"id":"0215073e-0217-5f59-97b2-f79f31df0b56","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-lipid-iron","predicate":"increases_tested","statement":"Cucurbitacin B increased lipid ROS, malondialdehyde and measured ferrous iron while lowering glutathione in H358 and A549 experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"f6344b10-076e-58ae-bb8a-6e328d6909de","mechanism_event_label":"Iron and antioxidant balance changed together with membrane damage.","subject":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"object":{"id":"cec3de0d-ba4c-55ab-863c-1b16e4c38e61","slug":"human-cucurbitacin-lipid-peroxidation","display_name":"Human cancer-cell lipid peroxidation during cucurbitacin B exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f6344b10-076e-58ae-bb8a-6e328d6909de","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-lipid-iron-event","event_type":"observed_relationship","label":"Iron and antioxidant balance changed together with membrane damage.","description":"Cucurbitacin B increased lipid ROS, malondialdehyde and measured ferrous iron while lowering glutathione in H358 and A549 experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cec3de0d-ba4c-55ab-863c-1b16e4c38e61","slug":"human-cucurbitacin-lipid-peroxidation","display_name":"Human cancer-cell lipid peroxidation during cucurbitacin B exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"59d6d1cd-df32-5b58-b950-3188bc7b95d6","slug":"iron-ii","display_name":"Ferrous iron","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human NSCLC experiments; concentration and duration unavailable in accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Associated readouts do not by themselves order iron release, glutathione loss and lipid oxidation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Iron and antioxidant balance changed together with membrane damage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin B targets STAT3 to induce ferroptosis in non-small cell lung cancer. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38950838/ · DOI 10.1016/j.ejphar.2024.176805","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0f06c48d-db08-5186-b56a-3e08f3d08972","evidence_kind":"source_excerpt","locator":"Lines 260-266","start_line":260,"end_line":266,"excerpt":"## cucurbitacin-b-lipid-iron\nIron and antioxidant balance changed together with membrane damage.\nCucurbitacin B increased lipid ROS, malondialdehyde and measured ferrous iron while lowering glutathione in H358 and A549 experiments.\nModel: Human NSCLC experiments; concentration and duration unavailable in accessed abstract.\nLimitations: Associated readouts do not by themselves order iron release, glutathione loss and lipid oxidation.\nEvidence access: Primary abstract\nCucurbitacin B targets STAT3 to induce ferroptosis in non-small cell lung cancer. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38950838/ · DOI 10.1016/j.ejphar.2024.176805","model_system":"Human NSCLC experiments; concentration and duration unavailable in accessed abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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