{"id":"011282a6-b591-5fad-b9b3-a1920f327f22","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-slc19a3-not-biotin-transporter","predicate":"does-not-increase","statement":"Wild-type SLC19A3 did not confer detectable biotin uptake, whereas the SLC5A6 positive control did.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"32885797-a5e5-59a9-a61a-a3168a532def","mechanism_event_label":"The name biotin-responsive disease does not make SLC19A3 a biotin carrier.","subject":{"id":"4eba176e-17c8-5515-920a-df5d48a40486","slug":"slc19a3","display_name":"Human thiamine transporter 2 / SLC19A3","entity_type_key":"protein"},"object":{"id":"d25f4daa-5529-568f-b319-7c30e444914b","slug":"cellular-biotin-uptake","display_name":"Cellular biotin uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"32885797-a5e5-59a9-a61a-a3168a532def","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-slc19a3-not-biotin-transporter-event","event_type":"biochemical_relationship","label":"The name biotin-responsive disease does not make SLC19A3 a biotin carrier.","description":"Wild-type SLC19A3 did not confer detectable biotin uptake, whereas the SLC5A6 positive control did.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"tested substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3bd59e38-e6b1-5223-b4de-faa33835f5bc","slug":"slc5a6","display_name":"Human sodium-dependent multivitamin transporter / SLC5A6","entity_type_key":"protein"},"role":"positive transport control","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4eba176e-17c8-5515-920a-df5d48a40486","slug":"slc19a3","display_name":"Human thiamine transporter 2 / SLC19A3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d25f4daa-5529-568f-b319-7c30e444914b","slug":"cellular-biotin-uptake","display_name":"Cellular biotin uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Distinguishes thiamine transport from biotin transport; no shared-substrate mechanism demonstrated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence-scope","value_text":"Caco-2 and MDCK epithelial models","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_locator","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_spans","value_text":"[{\"source_document\": \"artifacts/thiamine_transport_sources/subramanian-2006-biotin-specificity-source-record.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1656}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human transporter constructs in epithelial cell lines; uptake and surface targeting.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Tested cell models; clinical biotin-response mechanism remains unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Thiamine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human proteins in human and canine cell lines","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The name biotin-responsive disease does not make SLC19A3 a biotin carrier.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[subramanian-2006-biotin-specificity] Biotin-responsive basal ganglia disease-linked mutations inhibit thiamine transport via hTHTR2: biotin is not a substrate for hTHTR2 (2006). https://pubmed.ncbi.nlm.nih.gov/16790503/ DOI: 10.1152/ajpcell.00105.2006","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Caco-2 and MDCK epithelial models","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"559b08fe-fc91-5508-afef-3ff88caa8bc3","evidence_kind":"source_excerpt","locator":"Lines 294-307","start_line":294,"end_line":307,"excerpt":"### b1-slc19a3-not-biotin-transporter\nWild-type SLC19A3 did not confer detectable biotin uptake, whereas the SLC5A6 positive control did.\nCondition category: normal\nnutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The name biotin-responsive disease does not make SLC19A3 a biotin carrier.\norganism: Human proteins in human and canine cell lines\ntissue_or_cell_type: Caco-2 and MDCK epithelial models\nexperimental_model: Human transporter constructs in epithelial cell lines; uptake and surface targeting.\nlimitations: Tested cell models; clinical biotin-response mechanism remains unresolved.\ncross_nutrient: Distinguishes thiamine transport from biotin transport; no shared-substrate mechanism demonstrated.\nevidence_spans: [{\"source_document\": \"artifacts/thiamine_transport_sources/subramanian-2006-biotin-specificity-source-record.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1656}]\nevidence_locator: Abstract\nevidence-scope: Caco-2 and MDCK epithelial models\n[subramanian-2006-biotin-specificity] Biotin-responsive basal ganglia disease-linked mutations inhibit thiamine transport via hTHTR2: biotin is not a substrate for hTHTR2 (2006). https://pubmed.ncbi.nlm.nih.gov/16790503/ DOI: 10.1152/ajpcell.00105.2006","model_system":"Human transporter constructs in epithelial cell lines; uptake and surface targeting.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [subramanian-2006-biotin-specificity] Biotin-responsive basal ganglia disease-linked mutations inhibit thiamine transport via hTHTR2: biotin is not a substrate for hTHTR2 (2006). https://pubmed.ncbi.nlm.nih.gov/16790503/ DOI: 10.1152/ajpcell.00105.2006","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"158d2c03-ac8c-589f-8270-c468165ae346","stable_key":"import-46d15d9e-d3b5-544d-ba01-b785aa3e4f42","title":"Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f376512fb3310141315548015b387833e3af45146e73fb73cd02cee20e4ddb9c","revision_id":"53bc5eda-dec8-58cc-a56f-a9eb4ab036ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}