{"id":"00fb5941-8781-5ef9-8fa9-e0edc0906c00","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-cd38-inhibition","predicate":"inhibits","statement":"Luteolin inhibited human CD38 at an IC50 below 10 micromolar in vitro.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"784fe4c1-79c5-5d75-9bda-6e4186d8dc0e","mechanism_event_label":"One experimentally inhibited target consumes NAD-related substrates.","subject":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"object":{"id":"fddfde71-6536-5ac2-82cd-5942ad68b116","slug":"cd38","display_name":"Human CD38","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"784fe4c1-79c5-5d75-9bda-6e4186d8dc0e","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-cd38-inhibition-event","event_type":"observed_relationship","label":"One experimentally inhibited target consumes NAD-related substrates.","description":"Luteolin inhibited human CD38 at an IC50 below 10 micromolar in vitro.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fddfde71-6536-5ac2-82cd-5942ad68b116","slug":"cd38","display_name":"Human CD38","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human CD38 enzyme assay with docking.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Docking does not prove a binding pose; no human NAD increase or longevity effect established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"One experimentally inhibited target consumes NAD-related substrates.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Flavonoids as inhibitors of human CD38. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21641214/ · DOI 10.1016/j.bmcl.2011.05.022","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7f046a5f-8825-5698-bafe-891d7a121db8","evidence_kind":"source_excerpt","locator":"Lines 276-282","start_line":276,"end_line":282,"excerpt":"## luteolin-cd38-inhibition\nOne experimentally inhibited target consumes NAD-related substrates.\nLuteolin inhibited human CD38 at an IC50 below 10 micromolar in vitro.\nModel: Human CD38 enzyme assay with docking.\nLimitations: Docking does not prove a binding pose; no human NAD increase or longevity effect established.\nEvidence access: Primary abstract\nFlavonoids as inhibitors of human CD38. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21641214/ · DOI 10.1016/j.bmcl.2011.05.022","model_system":"Human CD38 enzyme assay with docking.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"fd6ea0b1cef8d1c9965270494bfa815de14f679044f0b5d5d7bbf4904630ad76","revision_id":"c9a3dd80-c7e6-5fb4-bc91-8db265e7c8de","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}