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(1992). https://pubmed.ncbi.nlm.nih.gov/1502165/ DOI: 10.1073/pnas.89.16.7536","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified/recombinant IRE-BP and cultured cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4c944c61-dd74-541a-9f88-02f511d3d139","evidence_kind":"source_excerpt","locator":"Lines 667-678","start_line":667,"end_line":678,"excerpt":"### iron-irp1-switch\nManipulations of the IRE-binding protein Fe-S cluster reciprocally altered RNA-binding and aconitase activities.\nCondition category: normal\nnutrient_topic: Iron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: One protein can act as an enzyme or an iron-responsive RNA-binding regulator, depending on its cluster state.\norganism: Mammalian IRE-binding protein systems\ntissue_or_cell_type: Purified/recombinant IRE-BP and cultured cells\nexperimental_model: Iron-sulfur manipulation and enzyme/RNA-binding assays\nlimitations: IRP1/aconitase switch; no assertion that IRP2 has the same catalytic switch.\nexposure: Iron and Fe-S cluster manipulation\nevidence_span: {\"source_cache\": \"artifacts/iron-research/1502165.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1fc2a7abe3fbac12a5196a163c8d6f21f5847bd89c049958dfb4055302a14a56\", \"start_char\": 0, \"end_char\": 1583, \"text_sha256\": \"1fc2a7abe3fbac12a5196a163c8d6f21f5847bd89c049958dfb4055302a14a56\"}\n[iron-p1502165] Reciprocal control of RNA-binding and aconitase activity in the regulation of the iron-responsive element binding protein: role of the iron-sulfur cluster. 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