{"id":"009dd9d6-7f93-5fee-bad7-0df357f1a738","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-gls2-gsh","predicate":"supports_tested","statement":"Increasing GLS2 supported higher reduced-glutathione levels, lower reactive oxygen species and protection from peroxide-induced apoptosis in the reported cell experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"95571f79-271f-5ffc-8db4-e19da2f5053c","mechanism_event_label":"The same supply route fed antioxidant chemistry as well as energy metabolism.","subject":{"id":"6981fb90-5bc3-5c2a-9f22-f1974e287f38","slug":"gls2","display_name":"Human glutaminase 2 / GLS2","entity_type_key":"protein"},"object":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"95571f79-271f-5ffc-8db4-e19da2f5053c","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-gls2-gsh-event","event_type":"observed_relationship","label":"The same supply route fed antioxidant chemistry as well as energy metabolism.","description":"Increasing GLS2 supported higher reduced-glutathione levels, lower reactive oxygen species and protection from peroxide-induced apoptosis in the reported cell experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6981fb90-5bc3-5c2a-9f22-f1974e287f38","slug":"gls2","display_name":"Human glutaminase 2 / GLS2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human-cell GLS2/p53 and oxidative-stress experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cysteine, glycine and synthesis machinery remain necessary; glutamate is not established as the universal limiting substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The same supply route fed antioxidant chemistry as well as energy metabolism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20378837/ · DOI 10.1073/pnas.1001006107","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"39889251-1930-5a53-8b52-c71001fb8374","evidence_kind":"source_excerpt","locator":"Lines 226-232","start_line":226,"end_line":232,"excerpt":"## glutamate-gls2-gsh\nThe same supply route fed antioxidant chemistry as well as energy metabolism.\nIncreasing GLS2 supported higher reduced-glutathione levels, lower reactive oxygen species and protection from peroxide-induced apoptosis in the reported cell experiments.\nModel: Human-cell GLS2/p53 and oxidative-stress experiments.\nLimitations: Cysteine, glycine and synthesis machinery remain necessary; glutamate is not established as the universal limiting substrate.\nEvidence access: Primary abstract\nGlutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20378837/ · DOI 10.1073/pnas.1001006107","model_system":"Human-cell GLS2/p53 and oxidative-stress experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3da83bd75fddeb96d17af72d7e2ae04344779957650f266521f6c8ebd59fb54f","revision_id":"55196c72-94e4-540a-a869-81d35647b04a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}