{"id":"00296a60-bdbd-5a84-be55-8e03b599da13","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-hpdl-hma","predicate":"produces","statement":"HPDL produced 4-hydroxymandelate in human cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8e5bd6a8-ec82-5b8c-af8b-85a0cdd0c768","mechanism_event_label":"A separate enzyme helps make the head of the CoQ molecule.","subject":{"id":"04a3616a-be0b-5217-bca3-2438680fe395","slug":"hpdl","display_name":"Human hydroxyphenylpyruvate dioxygenase-like protein / HPDL","entity_type_key":"protein"},"object":{"id":"4d1110c4-dcaf-5522-9f71-a8c2c16cd62d","slug":"4-hydroxymandelate","display_name":"4-Hydroxymandelate / 4-HMA","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"8e5bd6a8-ec82-5b8c-af8b-85a0cdd0c768","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-hpdl-hma-event","event_type":"biochemical_relationship","label":"A separate enzyme helps make the head of the CoQ molecule.","description":"HPDL produced 4-hydroxymandelate in human cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9e57c89c-2e87-5bc7-b2b9-29b122942fdd","slug":"4-hydroxyphenylpyruvate","display_name":"4-Hydroxyphenylpyruvate","entity_type_key":"small_molecule"},"role":"precursor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"899c7ab0-6f81-5b38-a6bd-fbb33d66ac8d","slug":"oxygen","display_name":"Molecular oxygen","entity_type_key":"small_molecule"},"role":"isotope_tracer","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"04a3616a-be0b-5217-bca3-2438680fe395","slug":"hpdl","display_name":"Human hydroxyphenylpyruvate dioxygenase-like protein / HPDL","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4d1110c4-dcaf-5522-9f71-a8c2c16cd62d","slug":"4-hydroxymandelate","display_name":"4-Hydroxymandelate / 4-HMA","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coq10-research/34471290.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a3cbcc69203ace49d0451e55c2d4040109e16facb575f4d66a0396d375ba8956\", \"start_char\": 0, \"end_char\": 1028, \"text_sha256\": \"a3cbcc69203ace49d0451e55c2d4040109e16facb575f4d66a0396d375ba8956\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Oxygen-isotope metabolomics and enzyme perturbation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"18O2 labeling and HPDL activity","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cellular pathway; no evidence that additional tyrosine treats every CoQ deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coq10","display_name":"Coenzyme Q10 / CoQ10 redox system","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human cell lines","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A separate enzyme helps make the head of the CoQ molecule.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coq10-p34471290] The polar oxy-metabolome reveals the 4-hydroxymandelate CoQ10 synthesis pathway. (2021). https://pubmed.ncbi.nlm.nih.gov/34471290/ DOI: 10.1038/s41586-021-03865-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Tyrosine-derived CoQ head-group precursor","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"df012cc6-6eac-5938-a99f-5507d4e54418","evidence_kind":"source_excerpt","locator":"Lines 164-175","start_line":164,"end_line":175,"excerpt":"### coq10-hpdl-hma\nHPDL produced 4-hydroxymandelate in human cells.\nCondition category: normal\nnutrient_topic: Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A separate enzyme helps make the head of the CoQ molecule.\norganism: Human cell lines\ntissue_or_cell_type: Tyrosine-derived CoQ head-group precursor\nexperimental_model: Oxygen-isotope metabolomics and enzyme perturbation\nlimitations: Cellular pathway; no evidence that additional tyrosine treats every CoQ deficiency.\nexposure: 18O2 labeling and HPDL activity\nevidence_span: {\"source_cache\": \"artifacts/coq10-research/34471290.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a3cbcc69203ace49d0451e55c2d4040109e16facb575f4d66a0396d375ba8956\", \"start_char\": 0, \"end_char\": 1028, \"text_sha256\": \"a3cbcc69203ace49d0451e55c2d4040109e16facb575f4d66a0396d375ba8956\"}\n[coq10-p34471290] The polar oxy-metabolome reveals the 4-hydroxymandelate CoQ10 synthesis pathway. 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