{"id":"001f36cb-f6e5-5e7b-86c4-86c676125e8b","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-cresol-membranes","predicate":"impairs","statement":"Exogenous p-cresol impaired susceptible Gram-negative bacterial membrane integrity; Gram-positive organisms were generally more tolerant.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c210b09e-abf5-5e92-9e2b-eceadf5e29d4","mechanism_event_label":"A microbial breakdown product affects other community members.","subject":{"id":"f08177fb-b34e-5589-a96c-46066ef57131","slug":"p-cresol","display_name":"Para-cresol / p-cresol","entity_type_key":"small_molecule"},"object":{"id":"4cc77515-b5d7-59f8-9c1d-5aebf3fc5d69","slug":"gram-negative-bacterial-membrane-integrity","display_name":"Gram-negative bacterial membrane integrity under p-cresol exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c210b09e-abf5-5e92-9e2b-eceadf5e29d4","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-cresol-membranes-event","event_type":"observed_relationship","label":"A microbial breakdown product affects other community members.","description":"Exogenous p-cresol impaired susceptible Gram-negative bacterial membrane integrity; Gram-positive organisms were generally more tolerant.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f08177fb-b34e-5589-a96c-46066ef57131","slug":"p-cresol","display_name":"Para-cresol / p-cresol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4cc77515-b5d7-59f8-9c1d-5aebf3fc5d69","slug":"gram-negative-bacterial-membrane-integrity","display_name":"Gram-negative bacterial membrane integrity under p-cresol exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bcfef85f-831d-5439-ba51-1aef4b090441","slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Defined bacterial exposure and membrane-integrity assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Susceptibility varies among organisms; no human systemic toxicity threshold is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Tyrosine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A microbial breakdown product affects other community members.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Para-cresol production by Clostridium difficile affects microbial diversity and membrane integrity of Gram-negative bacteria. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30208103/ · DOI 10.1371/journal.ppat.1007191","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a154cf07-8a1f-5d08-b708-d13a45ff7a96","evidence_kind":"source_excerpt","locator":"Lines 356-362","start_line":356,"end_line":362,"excerpt":"## l-tyrosine-cresol-membranes\nA microbial breakdown product affects other community members.\nExogenous p-cresol impaired susceptible Gram-negative bacterial membrane integrity; Gram-positive organisms were generally more tolerant.\nModel: Defined bacterial exposure and membrane-integrity assays.\nLimitations: Susceptibility varies among organisms; no human systemic toxicity threshold is inferred.\nEvidence access: Primary abstract\nPara-cresol production by Clostridium difficile affects microbial diversity and membrane integrity of Gram-negative bacteria. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30208103/ · DOI 10.1371/journal.ppat.1007191","model_system":"Defined bacterial exposure and membrane-integrity assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"12917df2-c6e0-5b61-850f-dbff6d4b4d30","stable_key":"import-63ce713e-6aea-59f6-9896-ca30e010b2ce","title":"L-Tyrosine: catecholamines, thyroid chemistry, pigment, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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